Article

Optimization of lipase production using differential evolution

Biotechnology and Bioprocess Engineering (Impact Factor: 1.22). 04/2010; 15(2):254-260. DOI: 10.1007/s12257-009-0163-3

ABSTRACT Differential Evolution (DE) coupled with Response Surface Methodology (RSM) has been used for the optimization of extracellular
lipolytic enzyme production by Rhizopus oryzae NRRL 3562 through sold state fermentation. The input space of the experimentally validated RSM-model was optimized using
a novel Differential Evolution approach (DE), which works based on the natural selection and survival of the fittest concepts
of the biological world. The maximum lipase activity of 96.52 U/gds was observed with the DE stated optimum values of 35.59°C,
1.50, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively. The optimal levels
of control parameters namely number of population, generations, crossover operator, and mutation constant were equal to 20,
50, 0.6, and 0.20, respectively. The developed model and its optimization are generic in nature and thus appear to be useful
for the design and scale-up of the extracellular lipase production by R. oryzae NRRL 3562 through solid state fermentation.

KeywordsOptimization-lipase-solid state fermentation-
Rhizopus oryzae NRRL 3562-Differential Evolution (DE)

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Available from: Vijay Kumar Garlapati, Aug 21, 2015
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    • "Extracellular lipase production by Rhizopus oryzae NRRL 3562 was optimized by response surface methodology (RSM) through SSF using wheat bran. The optimum values of 35.6 • C, 1.5, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively led to a maximum activity of 96.52 U/g (Garlapati and Banerjee, 2010). The effects of twelve medium components on wheat bran for A. niger lipase production were preliminary screened using Plackett–Burman (PB) design and six components (glucose, yeast extract, peptone, NaH 2 PO 4 , KH 2 PO 4 and water content) were optimized, which yielded an activity of 33.03 U/ml (Contesini et al., 2009). "
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    • "Lipase was produced through solid state fermentation using wheat bran as a substrate by using Rhizopus oryzae NRRL 3562 strain maintained on potato dextrose medium [9]. Immobilized lipase was prepared by using activated silica gel (60-120 mesh) through covalent immobilization [10]. "
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