Optimization of lipase production using differential evolution

Biotechnology and Bioprocess Engineering (Impact Factor: 1.11). 04/2010; 15(2):254-260. DOI: 10.1007/s12257-009-0163-3


Differential Evolution (DE) coupled with Response Surface Methodology (RSM) has been used for the optimization of extracellular
lipolytic enzyme production by Rhizopus oryzae NRRL 3562 through sold state fermentation. The input space of the experimentally validated RSM-model was optimized using
a novel Differential Evolution approach (DE), which works based on the natural selection and survival of the fittest concepts
of the biological world. The maximum lipase activity of 96.52 U/gds was observed with the DE stated optimum values of 35.59°C,
1.50, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively. The optimal levels
of control parameters namely number of population, generations, crossover operator, and mutation constant were equal to 20,
50, 0.6, and 0.20, respectively. The developed model and its optimization are generic in nature and thus appear to be useful
for the design and scale-up of the extracellular lipase production by R. oryzae NRRL 3562 through solid state fermentation.

KeywordsOptimization-lipase-solid state fermentation-
Rhizopus oryzae NRRL 3562-Differential Evolution (DE)

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Available from: Vijay Kumar Garlapati,
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    • "Lipase was produced using wheat bran as a substrate through solid-state fermentation [18]. Lipase immobilization was carried out using covalent attachment technique using activated silica as immobilization matrix and was explained in our previous work [19] "
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    ABSTRACT: Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, and were found to be 310 mM and 0.10 mmol h−1 g−1, respectively.
    09/2013; Article ID 451652(2013):9 pages. DOI:10.1155/2013/451652
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    • "Extracellular lipase production by Rhizopus oryzae NRRL 3562 was optimized by response surface methodology (RSM) through SSF using wheat bran. The optimum values of 35.6 • C, 1.5, 5.28, and 4.83 days for temperature, liquid to solid ratio, pH, and incubation time respectively led to a maximum activity of 96.52 U/g (Garlapati and Banerjee, 2010). The effects of twelve medium components on wheat bran for A. niger lipase production were preliminary screened using Plackett–Burman (PB) design and six components (glucose, yeast extract, peptone, NaH 2 PO 4 , KH 2 PO 4 and water content) were optimized, which yielded an activity of 33.03 U/ml (Contesini et al., 2009). "
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    ABSTRACT: Bioconversion of agricultural residues for lipase production as well as other value added products would hold a prominent position in future biotechnologies, mainly because of its eco friendliness and flexibility to both developing and developed countries. Several residues such as straw, bran, oil cakes, among others attract increasing attention as abundant and cheap renewable feedstock. Many researchers considered improvement of substrate composition, physical parameters (temperature, pH, moisture content and particle size), inoculum concentration, and substrate porosity to upgrade and valorize these bioproducts. Several species of fungi, yeast and bacteria have been used in utilizing the agricultural residues through fermentation techniques due to their ability to grow on particle surfaces as sources of carbon and energy, and produce important industrial enzymes including lipases. This review provides an overview of the present status on the utilization of renewable residues in the form of solid- and liquid-state and their characteristics for production of lipases using different microbial systems.
    Resources Conservation and Recycling 01/2012; 58:36–44. DOI:10.1016/j.resconrec.2011.10.007 · 2.56 Impact Factor
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    • "Lipase was produced through solid state fermentation using wheat bran as a substrate by using Rhizopus oryzae NRRL 3562 strain maintained on potato dextrose medium [9]. Immobilized lipase was prepared by using activated silica gel (60-120 mesh) through covalent immobilization [10]. "
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    ABSTRACT: In the present study, optimization of immobilized lipase mediated solvent-free synthesis of octyl acetate by transesterification has been carried out based on the experimentally validated RSM model. The effect of tansesterification variables namely alcohol molarity, reaction time, temperature, Immobilized lipase amount on molar conversion (%) was investigated. A maximum molar conversion of 92.92% was obtained with the GA parameters of tournament selection of size two, uniform crossover probability (P c) of 0.5, bit-wise mutational probability (P m) of 0.0029, population size of 80 and maximum number of generations of 382. The optimal values of the transesterification variables were found to be 2.09 M octanol in vinyl acetate, 12.61 h, 32°C and 26.01 U for alcohol molarity, reaction time, temperature and amount of Immobilized enzyme respectively
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