Article

Mise en évidence des cellules inflammatoires dans le sperme

Andrologie 01/1995; 5(4):548-554. DOI: 10.1007/BF03034541

ABSTRACT Une concentration élevée de cellules rondes dans un sperme réduit la fécondance. Ces cellules peuvent être des cellules germinales
immatures résultant d'une souffrance testiculaire accompagnée par la tératospermie, ou des polynucléaires s'ils sont peroxydases
positifs. Il arrive que ni la tératospermie, ni la réaction à la peroxydase n'expliquent le nombre élevé de ces cellules.
Pour ces spermes, nous avons utilisé la coloration de l'estérase non spécifique pour la recherche des macrophages, des plasmocytes
et des lymphocytes. Ces trois types de cellules ont été retrouvés dans certains spermes non leucospermiques. Ce qui reflète
une infection chronique en cours d'évolution. Les macrophages, les lymphocytes et les plasmocytes ont également été retrouvés
dans certains spermes leucospermiques. La présence des macrophages est corrélée avec celle des plasmocytes (r=0,65 — p=0,01)
et des lymphocytes (r=0,56 — p=0,05). Entre les plasmocytes et les lymphocytes la corrélation est plus importante (r=0,91
— p=0,001).
A high level of round cells in semen decreases the fertility capacity. These cells can be either immature germinal cells from
testicular disorders characterized by teratospermia, or polymorphonuclear cells if they are peroxidase positive. However it
often happens that neither teratospermia nor peroxidase reaction can explain the high proportion of round cells. For these
semen we have used the nonspecific esterase staining to search for macrophages, plasma-cells and lymphocytes. These three
types of cells were detected in certain non-leucospermic semen which reflects a chronic infection in development. Also these
cells, macrophages, plasma-cells and lymphocytes were found in some leucospermic semen samples. If we considere the populations
leucospermic and non leucospermic together, macrophages are correlated — with plasma-cells (r=0,65 — p=0,01) and with lymphocytes
(r=0,56 — p=0,05). The correlation between plasma-cells and lymphocytes was more important (r=0,91 — p=0,001).

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