Characterisation of Shake Flasks for Cultivation of Animal Cell Cultures
ABSTRACT This study investigated the oxygen transfer processes and general correlations between culture performance and the operating
conditions for shake flask fermentations of animal cell cultures. This involved both the online measurement of the oxygen
transfer rate and the continuous recording of measurements for the dissolved oxygen concentration in the shake flask.
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ABSTRACT: The oxygen transfer rate (OTR) is the most suitable measurable parameter to quantify the physiological state of a culture of aerobic microorganisms since most metabolic activities depend on oxygen consumption. Online measurement of the oxygen transfer rate in stirred bioreactors is state of the art although technically difficult. However, the online determination of the oxygen transfer rate in shaking bioreactors under sterile conditions has not been possible until recently. A newly developed measuring device eliminates this deficit. Extremely useful information about cultivating conditions and the physiological state of microorganisms can be gained in early stages of research and bioprocess development from many reactors operated in parallel.Biochemical Engineering Journal 04/2001; 7(2):157-162. · 2.58 Impact Factor