Article

Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot spring

Archives of Microbiology (impact factor: 1.43). 04/2012; 194(5):315-322. DOI:10.1007/s00203-011-0762-1 pp.315-322

ABSTRACT An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated
from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23T, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic
analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23T is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas
chromatography were iso-C15:0, iso-C17:0 and iso C17:1 ω9c. The major respiratory quinone was MK-8. The DNA G+C content was 66.9mol%. DNA–DNA hybridization measurements revealed
low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23T represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23T (=DSM 18496T; LMG 23756T).

KeywordsTaxonomy–New species–Sahara–Hot spring–Deinococcus–UV resistance

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Keywords

16S rRNA gene sequences
 
94.0% similarity values
 
aerobic cocci
 
chemotaxonomic characterization
 
closest neighbor
 
DNA G+C content
 
Gram-positive
 
KeywordsTaxonomy–New species–Sahara–Hot spring–Deinococcus–UV resistance
 
low DNA relatedness
 
major respiratory quinone
 
name Deinococcus sahariens sp
 
phenotypic
 
phylogenetic data
 
polyphasic taxonomy
 
predominant cellular fatty acids
 
Saharan water hot spring
 
thermophilic
 
type strain Deinococcus geothermalis DSM 11300