Brief History of the Tissue Bank, Charles University Hospital, Hradec Králové, Czech Republic

University Hospital
Cell and Tissue Banking (Impact Factor: 1.25). 02/2000; 1(1):17-25. DOI: 10.1023/A:1010128228619

ABSTRACT A brief history of the Tissue Bank (TB) of the University Hospital Hradec Krlov, Czech Republic, established by Dr. R. Klen in 1952 is presented. In Dr. Klen's original concept the TB was defined as a department specialised in the harvesting, processing, preservation, storage and distribution of various kinds of tissue for clinical and experimental practice. The first kinds of tissue collected in cadaveric donors were corneas, bone and skin. Xenogeneic cartilage and bone grafts were prepared at the same time. Later, preparation of soft connective tissues and chorion–amnion was introduced. During the first 15 years of activity a total of 11,443 grafts preserved by hypothermy at +4C or freezing in absence of cryoprotectants (–20C) were prepared. In the 60's freeze-drying of tissue grafts was introduced and the bank of cryopreserved cell lines was established. In the 80's cryopreservation of haematopoietic progenitor cells for clinical transplantations was started and the spectrum of tissue grafts was enlarged (xenogeneic pericardium and allogeneic specially treated dura mater for neurosurgical operations, pigskin for burn treatment, demineralised bone for parodontology and implantology). In the 90's human keratinocyte culture for treatment of burns and chronic skin defects was started. The human milk bank and organ bank co-operating with the Regional Transplantation Centre are component parts of the TB as well. The TB is an institutional member of the European Association of Tissue Banks and annually delivers approximately 1000 grafts that are used in University and county hospitals as well as in surgeons' private practices. Health insurance companies reimburse all grafts on a non-profit and tax-free basis.

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Available from: Pavel Mĕricka, Sep 26, 2015
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    • "The elective subject " Basis of Harvest and Preservation of Tissues " was established at the Charles University in Prague Medical Faculty Hradec Králové by authors in the academic year 2001/2002 both in Czech and English versions as an extension of teaching activities of the Tissue Bank University Hospital Hradec Králové existing before [1] [2]. The schedule of this subject was traditionally divided to lectures, practicals and a final seminar. "
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    ABSTRACT: ABSTRACT 1. Background The design and initial experience with the e-learning course: „Basis of Harvest and Preservation of Tissues“used as a support of an elective subject is presented The aim of the e-learning course was to enable the students to learn individually the theoretical principles of the subject and to present the gained knowledge at the final seminar. 2. Methods All functions of the course were operated in moodle, local application of the Charles University in Prague, Medical Faculty Hradec Králové. The course was divided into 3 main topics corresponding with topics of lectures: 1. Principles of tissue and organ donation, 2. Low temperature preservation of cells, tissues and organs, 3. Quality and safety assurance in practice of tissue and procurement establishments. A test consisting of 5 questions selected randomly from the bank of questions followed each topic. If the student answers correctly at least 3 questions he is allowed to pass to the next topic. The fourth topic „ Basic processes in the tissue establishment and principles of their validation“was added in the electronic version as a tool to repeating and enlargement of knowledge . The fifths topic was represented by a database for uploading theses presented by students at the final seminar. The final test consisted of 15 questions (5 ones from each basic topic). It was necessary to answer correctly at least 10 questions to receive a certificate of completing the course. 3. Results The course was put into operation during the summer term of the school- year 2012/2013. To the date 15 of September the total of 23 students enrolled (17,ie. all students of the elective subject in the Czech version, 2 students of this subject in the English version, 2 postgraduate students and 2 medical doctors).All enrolled students used the course for on - line learning, downloading, or printing course study materials. All undergraduate students were obliged to use it for preparation, consultation and presentation of seminar theses, ie 10 minute Power Point presentations followed by 5 minute discussion. Verification of the course is planed during the summer term of the school-year 2013/2014. 4. Conclusions The first experience showed that the presented e-learning course could serve as a useful support of the elective subject „Basis of Harvest and Preservation of Tissues“. It enlarged substantially the choice of study materials that can be regularly updated, so that the students receive the newest information. It can be attractive also for students that are not enrolled in the elective subject because of its limited capacity (not more than 20persons) The efficiency of such form of education must be, however a subject of further verification.