Article

Isolation and characterization of a new mannose-binding lectin gene fromTaxus media

Shanghai Jiao Tong University Plant Biotechnology Research Center, School of Agriculture and Biology 200030 Shanghai People’s Republic of China; Shanghai Jiao Tong University Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences and Technology 200030 Shanghai People’s Republic of China; Second Military Medical University School of Pharmacy 200433 Shanghai People’s Republic of China; Fudan University State Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center 200433 Shanghai People’s Republic of China; Fudan University School of Life Sciences, Morgan-Tan International Center for Life Sciences 200433 Shanghai People’s Republic of China
Journal of Biosciences (impact factor: 1.65). 04/2012; 29(4):399-407. DOI:10.1007/BF02712111 pp.399-407

ABSTRACT In this paper, we report the cloning and characterization of the first mannose-binding lectin gene from a gymnosperm plant
species,Taxus media. The full-length cDNA ofT. media agglutinin (TMA) consisted of 676 bp and contained a 432 bp open reading frame (ORF) encoding a 144 amino acid protein. Comparative
analysis showed that TMA had high homology with many previously reported plant mannose-binding lectins and thattma encoded a precursor lectin with a 26-aa signal peptide. Molecular modelling revealed that TMA was a new mannosebinding lectin
with three typical mannose-binding boxes like lectins from species of angiosperms. Tissue expression pattern analyses revealed
thattma is expressed in a tissue-specific manner in leaves and stems, but not in fruits and roots. Phylogenetic tree analyses showed
that TMA belonged to the structurally and evolutionarily closely related monocot mannose-binding lectin superfamily. This
study provides useful information to understand the molecular evolution of plant lectins.

0 0
 · 
0 Bookmarks
 · 
41 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

144 amino acid protein
 
26-aa signal peptide
 
432 bp open reading frame
 
characterization
 
cloning
 
fruits
 
gymnosperm plant
 
monocot mannose-binding lectin superfamily
 
new mannosebinding lectin
 
reported plant mannose-binding lectins
 
roots
 
structurally
 
thattma
 
thattma encoded
 
Tissue expression pattern analyses
 
TMA
 
useful information