Increase isoprene and monoterpene emissions after re-watering of droughted Quercus ilex seedlings

Biologia Plantarum (Impact Factor: 1.85). 06/2009; 53(2):351-354. DOI: 10.1007/s10535-009-0065-4


We followed the diurnal cycles of isoprenoid emissions from Quercus ilex seedlings under drought and after re-watering. We found that Quercus ilex, generally considered a non-isoprene emitter, also emitted isoprene although at low rates. The emission rates of isoprene
reached 0.37 ± 0.02 nmol m−2 s−1 in controls, 0.15 ± 0.03 nmol m−2 s−1 under drought and 0.35 ± 0.04 nmol m−2 s−1 after re-watering, while emission rates of monoterpenes reached 11.0 ± 3.0, 7.0 ± 1.0 and 23.0 ± 5.0 nmol m−2 s−1, respectively. Emission rates recovered faster after re-watering than photosynthetic rate and followed diurnal changes in
irradiance in controls and under drought, but in leaf temperature after re-watering.

Download full-text


Available from: Josep Penuelas,
  • Source
    • "As plant performance is coupled to the rate of transpiration and to net assimilation either of them may be regarded as the reference quantity. But, as already pointed out by Peñuelas et al. (2009) and by Loreto and Schnitzler (2010), droughtinduced responses in transpiration or net assimilation differ from responses in MT emissions. This general behaviour was also found here in all experiments and we therefore could not use either of them as a reference quantity. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Impacts of soil moisture on de novo monoterpene (MT) emissions from Holm oak, European beech, Scots pine, and Norway spruce were studied in laboratory experiments. The volumetric water content of the soil, Theta, was used as the reference quantity to parameterize the dependency of MT emissions on soil moisture and to characterize the severity of the drought. When Theta dropped from 0.4 m(3) x m(-3) to similar to 0.2 m(3) x m(-3) slight increases of de novo MT emissions were observed but with further progressing drought the emissions decreased to almost zero. In most cases the increases of MT emissions observed under conditions of mild drought were explainable by increases of leaf temperature due to lowered transpirational cooling. When Theta fell below certain thresholds, MT emissions decreased simultaneously with Theta and the relationship between Theta and MT emissions was approximately linear. The thresholds of Theta (0.044-0.19 m(3) x m(-3)) were determined, as well as other parameters required to describe the soil moisture dependence of de novo MT emissions for application in the Model of Emissions of Gases and Aerosols from Nature, MEGAN. A factorial approach was found appropriate to describe the impacts of Theta, temperature, and light. Temperature and Theta influenced the emissions largely independently from each other, and, in a similar manner, light intensity and Theta acted independently on de novo MT emissions. The use of Theta as the reference quantity in a factorial approach was tenable in predicting constitutive de novo MT emissions when Theta changed on a time scale of days. Empirical parameterization with Theta as a reference was only unsuccessful when soil moisture changed rapidly
    Biogeosciences 01/2015; 12(1):177-191. DOI:10.5194/bg-12-177-2015 · 3.98 Impact Factor
  • Source
    • "50 : up to a saturated max. 46 ; beyond saturation 60 High temperature :43 (up to a species specific max *358), then ;43 :46, 47 :47 :7 ; above optimum 61 Exposure to ozone ;44 due to degradation in atmosphere; : in intersticial tissue spaces 44, :as induced response 59 Variable, but overall :45; no effect or : 62 :48 :49 Variable, but overall :45 Adapted from Owen et al. (2013) 1 Blanch et al. 2007; 2 Blanch et al. (2012); 3 Rinnan et al. (2011); 4 Ormeno et al. (2009); 5 Possell et al. (2004); 6 Funk et al. (2006); 7, Seco et al. (2007); 8 Davison et al. (2008); 9 Raisanen et al. (2008); 10 Kim et al. (2005); 11 Street et al. (1997a); 12 Street et al. (1997b); 13 Hoertnagl et al. (2011); 14 Steinbrecher et al. (1997); 15 Tingey et al. (1981), 16 Sharkey and Loreto (1993), 17 Fang et al. (1996),18 Lerdau et al. (1997); 19 Brilli et al. (2007); 20 Lavoir et al. (2009); 21 Peñuelas et al. (2009); 22 Pegoraro et al. (2004); 23 Bertin & Staudt.(1996); 24 Ormeno et al. (2007); 25 Filella et al. (2009); 26 Brilli et al. (2011); 27 Llusia and Peñuelas (1998); 28 Piesik et al. (2011); 29 Schaub et al. (2010); 30 Staudt and Lhoutellier (2007); 31 Staudt et al. (2008); 32 Agelopoulos et al. (2000); 33 Hakola et al. (2001); 34 Bracho-Nunez et al. (2011); 35 Loreto et al. (2006); 36 Brilli et al. (2009); 37 Harley et al. (1996b); 38 Owen et al. (2002); 39 Tarvainen et al. (2005); 40 Staudt and Lhoutellier (2011); 41 Folkers et al. (2008); 42 Behnke et al. (2010); 43 Singsaas and Sharkey (2000); 44 Yuan et al. (2009); 45 Llusià et al. (2002);46 Guenther et al. (1995); 47 Vickers et al. (2009); 48 Bourtsoukidis et al. (2012); 49 Pellegrini et al. (2012); 50 Efthimiadou et al. (2010); 51 Murchie et al. (2009); 52 Dreyer et al. (1991); 53 Forrester et al. (2012); 54 Li and Lakso (2004); 55 Smetham (1995); 56 Bond (2000); 57 Chaves et al. (2003); 58 Zangerl et al. (2002); 59 Pinto et al.(2010); 60 Demmig-Adams et al. (2012); 61 Haldimann and Feller (2004); 62 Peñuelas et al. (1999) "
    [Show abstract] [Hide abstract]
    ABSTRACT: This study offers new insight and data in support of the “opportunist hypothesis”, which suggests that there might be a relationship between carotenoid and volatile isoprenoid production. Five species of volatile isoprenoid-emitting plants (Eucalyptus globulus, Eucalyptus gunnii, Mucuna pruriens, Lycopersicon esculentum and Quercus ilex) were exposed to a range of imposed and natural stress conditions over a period of a few weeks in order to generate different levels of isoprenoid production potential. Volatile isoprenoid emission potentials and carotenoid concentrations were measured in all species, and dimethylallyl diphosphate (DMAPP) concentrations were measured in E. globulus, E. gunnii, M. pruriens and L. esculentum. Generally, instantaneously emitted isoprenoid emission potentials were positively correlated with carotenoid concentrations, and were negatively correlated with DMAPP concentrations. In contrast, emission potentials of monoterpenes stored in tissue pools were negatively correlated with carotenoid concentrations, and positively correlated with DMAPP concentrations. Our results support the possibility of a link (either direct, e.g. via substrate availability, or indirect, e.g. via complementary functionality) between emission potential of the volatile isoprenoid compounds studied here, and carotenoid synthesis at time scales of days to weeks.
    Acta Physiologiae Plantarum 11/2013; 35(11). DOI:10.1007/s11738-013-1344-4 · 1.58 Impact Factor
  • Source
    • "However, regarding drought stress effects on BVOC emissions literature is conflicting. Upon imposing a drought stress, BVOC emissions are reported to be enhanced (Delfine et al., 2005; Blanch et al., 2009), reduced (Brilli et al., 2007; Fortunati et al., 2008; Lavoir et al., 2009; Pe nuelas et al., 2009), or increased followed by a decrease (Sharkey and Loreto, 1993; Bertin and Staudt, 1996; Orme no et al., 2007). Moreover, drought stress can alter BVOC species composition and/or induce new emissions depending on the level of stress (Niinemets, 2009). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Direct plant stress sensing is the key for a quantitative understanding of drought stress effects on biogenic volatile organic compound (BVOC) emissions. A given level of drought stress might have a fundamentally different effect on the BVOC emissions of different plants. For the first time, we continuously quantified the level of drought stress in a young potted beech (Fagus sylvatica L.) with a linear variable displacement transducer (LVDT) installed at stem level in combination with simulta- neous measurements of BVOC emissions and photosynthesis rates at leaf level. This continuous set of measurements allowed us to examine how beech alters its pattern of photosynthesis and carbon allo- cation to BVOC emissions (mainly monoterpenes, MTs) and radial stem growth during the development of drought stress. We observed an increasing-decreasing trend in the MT emissions as well as in the fraction of assimilated carbon re-emitted back into the atmosphere (ranging between 0.14 and 0.01%). We were able to link these dynamics to pronounced changes in radial stem growth, which served as a direct plant stress indicator. Interestingly, we detected a sudden burst in emission of a non-identified, non-MT BVOC species when drought stress was acute (i.e. pronounced negative stem growth). This burst might have been caused by a certain stress-related green leaf volatile, which disappeared immediately upon re-watering and thus the alleviation of drought stress. These results highlight that direct plant stress sensing creates opportunities to understand the overall complexity of stress-related BVOC emissions.
    Atmospheric Environment (1967) 09/2011; 45(30):5254-5259. DOI:10.1016/j.atmosenv.2011.06.075
Show more