Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor

Biotechnology and Bioprocess Engineering (Impact Factor: 1.11). 04/2007; 12(2):80-85. DOI: 10.1007/BF03028630


In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine
coupling method and utilized repeatedly by regeneration. The monoclonal antibody, ‘A18’, was selected and employed for the
high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 103 cells/ml or 50 cells were detected by the SPR biosensor.

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Available from: Sang-Do Ha, Apr 19, 2015
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