Article

Effect of starting xylose concentration on the microaerobic metabolism of Debaryomyces hansenii

Applied Biochemistry and Biotechnology (impact factor: 1.94). 04/2012; 101(1):15-29. DOI:10.1385/ABAB:101:1:15 pp.15-29

ABSTRACT Xylitol production by Debaryomyces hansenii NRRL Y-7426 was performed on synthetic medium varying the initial xylose concentration between 50 and 300 g/L. The experimental
results of these tests were used to investigate the effect of substrate level on xylose consumption by this yeast. Satisfactory
values of product yield on substrate (0.74–0.83 g/g) as well as volumetric productivity (0.481–0.694 g/L·h) were obtained
over a wide range of xylose levels (90–200 g/L), while a worsening of kinetic parameters took place at higher concentration,
likely due to a substrate inhibition phenomenon. The metabolic behavior of D. hansenii was studied, under these conditions, through a carbon material balance to estimate the fractions of xylose consumed by the
cell for different activities (xylitol production, biomass growth, and respiration) during the lag, exponential, and stationary
phases.

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    Article: Debaryomyces hansenii--an extremophilic yeast with biotechnological potential.
    Uta Breuer, Hauke Harms
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    ABSTRACT: We illuminate the ecological, physiological and genetic characteristics of the yeast Debaryomyces hansenii in the view of our belief that this metabolically versatile, non-pathogenic, osmotolerant and oleaginous microorganism represents an attractive target for fundamental and applied biotechnological research. To this end, we give a broad overview of extant biotechnological procedures using D. hansenii, e.g. in the manufacture of various foods, and propose research into the heterologous synthesis of a range of fine chemicals.
    Yeast 05/2006; 23(6):415-37. · 1.89 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

biomass growth
 
carbon material balance
 
D. hansenii
 
Debaryomyces hansenii NRRL Y-7426
 
different activities
 
higher concentration
 
initial xylose concentration
 
kinetic parameters
 
metabolic behavior
 
product yield
 
substrate inhibition phenomenon
 
synthetic medium varying
 
tests
 
volumetric productivity
 
Xylitol production
 
xylose
 
xylose consumption
 
xylose levels
 

Attilio Converti