Differentiation of sourdough yeast species by a novel species-specific PCR assay
ABSTRACT The objective of this study was to develop species-specific primer pairs based on the internal transcribed spacer region (ITS)
of the ribosome DNA for species identification of the frequently found sourdough yeast species. Species-specific primers were
designed by the alignment of sourdough yeast ITS sequences, which were then employed for PCR using the template DNA of sourdough
yeast strains. PCR primers were shown to be specific for the following species: Issatchenkia orientalis (Candida krusei), C. humilis, Kazachstania
exigua (C. holmii), Pichia anomala and P. subpelliculosa. Therefore, we conclude that our novel species-specific primers could be used to rapidly and accurately identify the most
frequent sourdough yeast species using a PCR-based assay.
KeywordsSpecies-specific PCR-Internal transcribed spacer-Sourdough yeast-Species identification