Article

Differentiation of sourdough yeast species by a novel species-specific PCR assay

World Journal of Microbiology and Biotechnology (impact factor: 1.53). 04/2012; 26(6):1087-1092. DOI:10.1007/s11274-009-0274-1 pp.1087-1092

ABSTRACT The objective of this study was to develop species-specific primer pairs based on the internal transcribed spacer region (ITS)
of the ribosome DNA for species identification of the frequently found sourdough yeast species. Species-specific primers were
designed by the alignment of sourdough yeast ITS sequences, which were then employed for PCR using the template DNA of sourdough
yeast strains. PCR primers were shown to be specific for the following species: Issatchenkia orientalis (Candida krusei), C. humilis, Kazachstania
exigua (C. holmii), Pichia anomala and P. subpelliculosa. Therefore, we conclude that our novel species-specific primers could be used to rapidly and accurately identify the most
frequent sourdough yeast species using a PCR-based assay.

KeywordsSpecies-specific PCR-Internal transcribed spacer-Sourdough yeast-Species identification

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Keywords

C. humilis
 
following species
 
found sourdough yeast species
 
frequent sourdough yeast species
 
internal transcribed spacer region
 
Issatchenkia orientalis
 
KeywordsSpecies-specific PCR-Internal transcribed spacer-Sourdough yeast-Species identification
 
novel species-specific primers
 
P. subpelliculosa
 
PCR primers
 
PCR-based assay
 
sequences
 
sourdough yeast
 
species identification
 
species-specific primer pairs
 
Species-specific primers
 
template DNA