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Improved Production of the Human Granulocyte-Macrophage Colony Stimulating Factor in Transgenic Arabidopsis thaliana Seeds Using a Dual Sorting Signal Peptide

Shanghai Jiao Tong University School of Agriculture and Biology 16 mailbox No. 800, Dong Chuan Road Shanghai 200240 The People’s Republic of China
Plant Molecular Biology Reporter (Impact Factor: 5.32). 27(4):462-468. DOI: 10.1007/s11105-009-0107-2

ABSTRACT The human granulocyte-macrophage colony stimulating factor (hGM-CSF) containing either an endoplasmic reticulum (ER) retention
signal or a phaseolin vacuolar sorting signal peptide was expressed in Arabidopsis thaliana under the control of a tissue-specific promoter, derived from the soybean α′ subunit of β-conglycinin. No significant differences
in recombinant hGM-CSF (rhGM-CSF) accumulation were detected between transgenic plants carrying either one of the two signal
peptides. Hybrid seed from crosses between single-copy transformants tailed with the ER retention signal tetrapeptide and
single-copy transformed plants tagged with a phaseolin four carboxy-terminal residues showed gene additive effects. The highest
expression level of rhGM-CSF was 0.05% of total soluble protein of immature siliques, indicating that the two signal peptides
functioned independently in the protein-sorting pathway. Additionally, TF-1 cell proliferation data demonstrated that rhGM-CSF
was biologically active.

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