Diallel analysis of maize inbred lines for carotenoids and grain yield

Euphytica (Impact Factor: 1.69). 01/2010; 182(3):395-404. DOI: 10.1007/s10681-011-0513-z

ABSTRACT Vitamin A deficiency causes xerophthalmia in preschool-aged children worldwide. The objective of this study was to estimate
the genetic parameters that would be useful in selecting parent plants for developing productive hybrids with higher levels
of provitamin A in the maize kernel. A complete 7×7 diallel mating scheme was used to generate 21 single-cross hybrids.
The F1 crosses and check hybrids were evaluated in complete block design across three different Brazilian environments, and carotenoid
content was analyzed by high performance liquid chromatography. General combining ability effects were significant for all
traits except α-carotene This result indicates that the contribution of the additive effect was more important for provitamin
A and other carotenoids and, consequently that there is high chance of improving this trait through recurrent selection methods.
Line 3 produced the highest level of kernel provitamin A among the inbred lines evaluated and also demonstrated the potential
to contribute to the development of genetic materials with a good performance for provitamin A. Inbred lines 1, 6, and 7 showed
a higher concentration of favorable alleles for grain yield, and inbred lines 3 and 6 exhibited a higher concentration of
favorable alleles for β-carotene. Hybrid 1×3 performed well in terms of provitamin A and grain yield and combination 2×3
was the best performer in terms of lutein content.

KeywordsCarotenoids–Provitamin A–Grain yield–Maize–General and specific combining ability

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    ABSTRACT: The purpose of this study was to investigate the adaptability and stability of carotenoids in maize cultivars inthe 2004/2005 growing season. Total carotenoids (TC), total carotenoids with provitamin A activity (Pro VA) (μg g-1) andgrain yield (kg ha-1) were quantified in 10 cultivars at five locations. The chemical analyses were conducted in a laboratoryof the EMBRAPA/CNPMS, in Sete Lagoas, Minas Gerais. The methodologies of Eberhart and Russell (1966), Lin and Binns(1988) and Rocha et al. (2005) were used to analyze adaptability and stability. In general, the linear regression modelproposed by Eberhart and Russell (1966) failed to fit the Pro VA contents in the evaluated cultivars satisfactorily. However,with regard to the TC levels, all different analysis methodologies of adaptability and stability rated hybrid BRS 2020 as anideal genotype with general adaptability.
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    ABSTRACT: Efforts to biofortify maize with provitamin A carotenoids have been successful, but the impact on vitamin A (VA) status has not been determined. We conducted two studies that investigated the bioefficacy of provitamin A carotenoids from maize and compared maize percentage and carotenoid concentrations on VA status in VA-depleted Mongolian gerbils (Meriones unguiculatus). Gerbils (n = 40/study) were fed a white maize diet 4 wk prior to treatment. In study 1, treatments (n = 10/group) included oil control, 60% high-beta-carotene maize, and beta-carotene or VA supplements (matched to high-beta-carotene maize). In study 2, gerbils were fed 30 or 60% orange or yellow maize diets. Gerbils were killed after 4 wk. In study 1, liver VA concentrations, compared with the high-beta-carotene maize group (0.25 +/- 0.15 micromol/g), were higher in the VA group (0.56 +/- 0.15 micromol/g, P < 0.05), lower in the control (0.10 +/- 0.04 micromol/g, P < 0.05), and did not differ in the beta-carotene group (0.25 +/- 0.08 micromol/g). Bioconversion was approximately 3 microg beta-carotene to 1 mug retinol (1.5 mol beta-carotene to 1 mol retinol). The liver beta-carotene content was greater in the high-beta-carotene maize group (26.4 +/- 6.0 nmol) than in the beta-carotene supplement group (14.1 +/- 6.0 nmol; P < 0.05). In study 2, the gerbils' VA status improved with increasing dietary beta-carotene. Liver VA in gerbils fed orange maize was greater than in those fed yellow maize, regardless of maize percentage (P < 0.05). Biofortified maize adequately maintained VA status in Mongolian gerbils and was as efficacious as beta-carotene supplementation. In populations consuming maize as a staple food, using orange instead of white maize could dramatically affect VA status.
    Journal of Nutrition 10/2006; 136(10):2562-7. · 4.23 Impact Factor


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Aug 21, 2014