Effects of diet on the fatty acid composition of body zones in sea bass, Dicentrarchus labrax L. larvae: a chemometric study

The University of Stirling
Marine Biology (Impact Factor: 2.39). 11/1995; 124(2):177-183. DOI: 10.1007/BF00347121


Larvae of the sea bass Dicentrachus labrax were fed four Artemia sp. diets for 28 d. Three were nauplii enriched with emulsions of polyunsaturated fatty acids, and the fourth nauplii enriched with baker's yeast. At the end of the experimental period, the fatty acids of the bodies, heads and eyes of the larvae were analysed. A multivariate statistical method (discriminant analysis, DA) applied to the data revealed anatomical as well as dietary fatty acid pattern-discrimination. We propose here the use of discriminant analysis as a pattern-recognition method that will help to integrate the fatty acid information obtained in nutritional studies.

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    • "It has been shown that these fatty acids contribute to proper development of the nervous system or sensory organs in fish larvae (Benitez-Santana et al., 2006; Navarro et al., 1995). "
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    ABSTRACT: We examined trophic positions and fatty acid concentrations of riverine, lacustrine, and aquaculture diet and fish in Austrian pre-alpine aquatic ecosystems. It was hypothesized that dietary fatty acid (FA) profiles largely influence the FA composition of the salmonids Salvelinus alpinus, Salmo trutta, and Oncorhynchus mykiss. We analyzed trophic positions using stable isotopes (δ15N) and tested for correlations with polyunsaturated fatty acid (PUFA) concentrations. Gut content analysis revealed benthos (rivers), pellets (aquaculture), and zooplankton (lakes) as the predominant diet source. Results of dorsal muscle tissues analysis showed that the omega-3 PUFA, docosahexaenoic acid (DHA; 22:6n−3), was the mostly retained PUFA in all fish of all ecosystems, yet with the highest concentrations in S. alpinus from aquaculture (mean: 20mg DHA/g dry weight). Moreover, we found that eicosapentaenoic acid (EPA; 20:5n−3) in fish of natural habitats (rivers, lakes) was the second most abundant PUFA (3–5mg/g DW), whereas aquaculture-raised fish had higher concentrations of the omega-6 linoleic acid (18:2n–6; 9–11mg/g DW) than EPA. In addition, PUFA patterns showed that higher omega-3/-6 ratios in aquacultures than in both riverine and lacustrine fish. Data of this pilot field study suggest that salmonids did not seem to directly adjust their PUFA to dietary PUFA profiles in either natural habitats or aquaculture and that some alterations of PUFA are plausible. Finally, we suggest that trophic positions of these freshwater salmonids do not predict PUFA concentrations in their dorsal muscle tissues. KeywordsAquatic food webs-Dietary fatty acids-Stable isotopes-Aquatic habitats-Fish
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    • "stinguish between dietary and non - dietary components . Some fatty acids are deposited in adipose tissue with little modification and in a predictable way ( Iverson et al . , 2004 ) . The specific FA patterns are passed from prey to predator near the bottom of the food web ( Sargent et al . , 1988 ; Fraser et al . , 1989 ; Graeve et al . , 1994 : Navarro et al . , 1995 ; St . John and Lund , 1996 ; Kirsch et al . , 1998 ) , determining the FA composition of higher predator levels ( Hooper et al . , 1973 ) and indicating the presence of specific prey in predator diets ( Colby et al . , 1993 ; Pond et al . , 1995 ; Raclot et al . , 1998 ) . Tracking of dietary components through the food web cannot be e"
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    • "The fatty acid nutrition of European sea bass has been investigated for broodstock (Bell et al., 1997; Navas et al., 1997, 1998; Bruce et al., 1999) and larval stages (Navarro et al., 1995, 1997). Special consideration has been given to elucidate the effect that different dietary fatty acid compositions have on egg and larval composition and quality, nevertheless the lowest levels of n-3 fatty acids indispensable for adequate growth of sea bass has yet to be determined (Oliva-Teles, 2000; Parpoura and Alexis, 2001). "
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