Article

Effect of RGDS and KRSR peptides immobilized on silk fibroin nanofibrous mats for cell adhesion and proliferation

Seoul National University Department of Biosystems & Biomaterials Science and Engineering Seoul 151-921 Korea; Seoul National University Intelligent Textile System Research Center Seoul 151-742 Korea; Seoul National University Reasearch Institute for Agriculture and Life Sciences Seoul 151-921 Korea
Macromolecular Research (impact factor: 1.15). 04/2012; 18(5):442-448. DOI:10.1007/s13233-010-0514-0 pp.442-448

ABSTRACT In this study, RGDS and KRSR peptides were immobilized onto electrospun silk fibroin (SF) nanofibrous mats by imide bond formation,
and the cell affinities were evaluated as an immobilized SF scaffold. The MTT assay showed that cell adhesion and spreading
of normal human dermal fibroblast (NHDF) occurs on SF nanofibrous mat with immobilized RGDS peptide in the early culture time
(within 2–4 h after seeding). On the other hand, the KRSR peptide was more effective on normal human osteoblasts (NHOst).
Therefore, the cell adhesion peptides RGDS and KRSR are effective in improving cell adhesion, spreading and proliferation
of specific cell types. Moreover, these effects depend on the peptide density. The performance of the SF nanofibrous mats
with immobilized peptides may be enhanced as a scaffold for specific uses.

Keywordssilk fibroin-nanofiber-scaffold-cell affinity-peptide immobilization

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Keywords

cell adhesion
 
cell adhesion peptides RGDS
 
cell affinities
 
electrospun silk fibroin
 
imide bond formation
 
immobilized peptides
 
immobilized RGDS peptide
 
immobilized SF scaffold
 
Keywordssilk fibroin-nanofiber-scaffold-cell affinity-peptide immobilization
 
MTT assay
 
normal human dermal fibroblast
 
normal human osteoblasts
 
SF
 
SF nanofibrous mat
 
SF nanofibrous mats
 
specific cell types