Topiramate and Vitamin E Modulate the Electroencephalographic Records, Brain Microsomal and Blood Antioxidant Redox System in Pentylentetrazol-Induced Seizure of Rats
ABSTRACT We investigated the effects of vitamin E and topiramate (TPM) administrations on pentylentetrazol (PTZ)–induced blood and
brain toxicity in rats. Forty rats were randomly divided into five equal groups. The first and second groups were used for
the control and PTZ groups, respectively. Fifty or 100mg TPM were administered to rats constituting the third and fourth
groups for 7days, respectively. The TPM and vitamin E combination was given to animals in the fifth group. At the end of
7days, all groups except the first received a single dose of PTZ. Blood and brain samples were taken at 3hrs after PTZ administration.
Lipid peroxidation levels of plasma, erythrocyte, brain cortex and brain microsomal fraction; nitric oxide levels of serum;
and the number of spikes and epileptiform discharges of the EEG were increased by PTZ administration. Plasma and brain vitamin
E concentration, erythrocyte glutathione peroxidase (GSH-Px) activity and latency to first spike of the EEG were decreased
by PTZ. Plasma lipid peroxidation levels in the third group and plasma and erythrocyte lipid peroxidation levels in the fifth
group were decreased compared to the second group, whereas brain vitamin C, vitamin E, erythrocyte GSH-Px and reduced glutathione
(GSH) values increased in the fifth group. Brain microsomal GSH levels and EEG records in the third, fourth and fifth groups
were restored by the TPM and vitamin E treatment. In conclusion, TPM and vitamin E seems to have protective effects on PTZ-induced
blood and brain toxicity by inhibiting free radicals and supporting the antioxidant redox system.
Article: Flavan-3-ol compounds prevent pentylenetetrazol-induced oxidative damage in rats without producing mutations and genotoxicity.[show abstract] [hide abstract]
ABSTRACT: Seizure disorder is a chronic condition in the brain that affects approximately 50 million people worldwide. Oxidative stress plays a crucial role in the pathophysiology of this disorder and can cause neuronal injury. Approximately one in three treated patients suffers from seizures regardless of pharmacological intervention, which results in oxidative damage. The present study aims to investigate the possible protective effect of antioxidant-rich Vitis Labrusca extract on pentylenetetrazol-induced oxidative damage in Wistar rats. Possible behavioral alterations, genotoxic and mutagenic effects of the extract were also evaluated. The results showed that V. labrusca extract provides a significant protective effect against oxidative damage to lipids and proteins induced by pentylenetetrazol in the cerebral cortex, cerebellum, hippocampus and liver of rats. Also, the extract did not alter locomotor behavior. Moreover, it was non-genotoxic and non-mutagenic. Our results suggest the possibility of using V. labrusca extract as a therapeutic agent to minimize neuronal damage associated with seizures.Neuroscience Letters 12/2012; · 2.11 Impact Factor