Topiramate and Vitamin E Modulate the Electroencephalographic Records, Brain Microsomal and Blood Antioxidant Redox System in Pentylentetrazol-Induced Seizure of Rats

Abstract

We investigated the effects of vitamin E and topiramate (TPM) administrations on pentylentetrazol (PTZ)–induced blood and
brain toxicity in rats. Forty rats were randomly divided into five equal groups. The first and second groups were used for
the control and PTZ groups, respectively. Fifty or 100mg TPM were administered to rats constituting the third and fourth
groups for 7days, respectively. The TPM and vitamin E combination was given to animals in the fifth group. At the end of
7days, all groups except the first received a single dose of PTZ. Blood and brain samples were taken at 3hrs after PTZ administration.
Lipid peroxidation levels of plasma, erythrocyte, brain cortex and brain microsomal fraction; nitric oxide levels of serum;
and the number of spikes and epileptiform discharges of the EEG were increased by PTZ administration. Plasma and brain vitamin
E concentration, erythrocyte glutathione peroxidase (GSH-Px) activity and latency to first spike of the EEG were decreased
by PTZ. Plasma lipid peroxidation levels in the third group and plasma and erythrocyte lipid peroxidation levels in the fifth
group were decreased compared to the second group, whereas brain vitamin C, vitamin E, erythrocyte GSH-Px and reduced glutathione
(GSH) values increased in the fifth group. Brain microsomal GSH levels and EEG records in the third, fourth and fifth groups
were restored by the TPM and vitamin E treatment. In conclusion, TPM and vitamin E seems to have protective effects on PTZ-induced
blood and brain toxicity by inhibiting free radicals and supporting the antioxidant redox system.