Article

Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

World Journal of Microbiology and Biotechnology (impact factor: 1.53). 04/2012; 27(9):2227-2230. DOI:10.1007/s11274-011-0679-5

ABSTRACT A rapid, sensitive and cost-effective method was developed for detection of foodborne pathogens, particularly Salmonella species. The method utilizes single stranded DNA (ssDNA) probes and non-functionalized gold nanoparticles to provide a colorimetric
assay for the detection of PCR amplified DNA. Different food samples were tested with the PCR-based colorimetric assay parallel
with the conventional culture method. The sensitivity and specificity of colorimetric assay was 89.15 and 99.04% respectively
with reference to conventional culture method. The total time required to detect the Salmonella spp. present in food samples by the developed method is less than 8h, including 6h incubation. It was observed that the
colorimetric assay was 10 times more sensitive than gel-based detection with the same concentration of DNA used for analysis.

KeywordsGold nanoparticles–PCR–
Salmonella
–ssDNA probes

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Keywords

colorimetric assay
 
conventional culture method
 
cost-effective method
 
detection
 
developed method
 
Different food samples
 
food samples
 
foodborne pathogens
 
gel-based detection
 
method utilizes single
 
non-functionalized gold nanoparticles
 
PCR amplified DNA
 
PCR-based colorimetric assay parallel
 
Salmonella spp
 
sensitive
 
ssDNA
 
total time