Article

Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)

College of biological and Environmental Engineering, Jiangsu University of Science and technology, Zhenjiang Jiangsu, 212018 China
Genes & genomics (Impact Factor: 0.5). 01/2011; 33(2):97-103. DOI: 10.1007/s13258-010-0124-4

ABSTRACT A full-length cDNA sequence encoding CaM from mulberry, which we designated MCaM-3 (GenBank accession No: GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed
that MCaM-3 is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric
point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium.
The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed
that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression
level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to
the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.

Keywords
Morus L. MCaM-3 gene–Molecular cloning–Gene expression

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