Stockplant Management for Optimized Rhizogenesis in Tectona grandis Stem Cuttings

New Forests (Impact Factor: 1.83). 12/2005; 31(1):91-96. DOI: 10.1007/s11056-004-7361-9


A 5-year stand of teak (Tectona grandis L. f.) was coppiced in 1999 and converted into a vegetative multiplication garden. Subsequently, three harvesting regimes
for the collection of single node stem cuttings were imposed: (1) once – in March (H1), (2) twice – in March and September (H2) and (3) three times in March, July and November (H3). Cuttings were treated basally with either:- T0 – control (6h in water), T1 – half the recommended dose of a mixture of IBA and thiamine (500ppm IBA +400ppm thiamine) or T2 – the full dose of the same mixture (1000ppm IBA +800ppm thiamine). Cuttings receiving IBA +thiamine rooted significantly
better than untreated cuttings, but even the best treatment only resulted in 38.3±3.8% rooting. This treatment produced
the greatest number of roots (5.2–12.1). The full dose treatment appears to have been supra-optimal. Rooting ability was also
affected by the frequency of stockplant pruning, with cuttings from stockplants pruned twice per year having the greatest
rooting percentage (27.8±3.8%) and the most roots (9.2±4.8). This bi-annual pruning (H2) resulted in the greatest number of rooted propagules (2.6 and 4.2 times more than H1 and H3, respectively). There was a significant interaction between Treatment×Pruning frequency. Bi-annual hedging of teak stockplants
is recommended for practical purposes, although further work is required to achieve commercially acceptable levels of rooting
from coppiced tree stumps.

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Available from: Shamim Akhtar Ansari, Nov 04, 2014
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    ABSTRACT: Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak (Tectona grandis L.). Higher number of shoots (6.17) per log was produced under natural light as compared to shade conditions. Forcing was also better in natural light as compared to shade in terms of shoot length, number of nodes or leaves. For rooting, 2–4 cm long softwood shoots were excised and treated with either indole-3-butyric acid (IBA) or α-naphthyl acetic acid (NAA) at 0, 1000, 2000 or 3000 μmol·L−1 each or with combinations (1000 + 1000, 2000 + 2000 or 3000 + 3000 μmol·L−1) and then placed in flat trays containing autoclaved sand at 25 ± 2°C in 16 h photoperiod at 35 μmol·m−2·s−1. After 28 days, softwood cuttings treated with IBA + NAA (3000 + 3000 μmol·L−1) had highest rooting percentage (89.3%) with 5.5 mean roots. Shoot apex and nodal explants of softwood cuttings were pretreated with 0.1% (w/v) ascorbic acid, boric acid, activated charcoal, citric acid, glutamine or polyvinylpolypyrollidone (PVP) for 24 h to remove phenolic compounds before surface disinfestation. Glutamine (Gl) and PVP were equally effective resulting in 60% establishment of shoot apices on MS medium supplemented with 10 μmol·L−1 6-benzylaminopurine (BAP) + 5 μmol·L−1 NAA. Using shoot apices, highest (42.80) number of multiple shoots with 54.33 mm shoot length were obtained on MS + BAP (8.8 μmol·L−1) + IBA (2 μmol·L−1) after 45 days. Shoots were successfully rooted and acclimatized to greenhouse conditions.
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