Étude du pouvoir antifongique de l’huile essentielle de cannelle
ABSTRACT L’huile essentielle de cannelle a fait l’objet d’études sur diverses souches fongiques pathogènes. L’huile essentielle a été
testée en utilisant trois techniques différentes : la technique de dilution en gélose pour la détermination des souches sensibles,
une microméthode de dilution en milieu liquide pour déterminer les concentrations minimales inhibitrices (CMI) de l’huile
essentielle sur la croissance des levures, une macrométhode de dilution en milieu liquide permettant l’évaluation de l’huile
essentielle sur la production de la masse mycélienne des souches filamenteuses. Les résultats obtenus montrent que les CMI
sont comprises entre 1/640 et 1/2560 pour les levures et entre 1/5000 et 1/10000 pour les moisissures. Sur la production mycélienne,
l’huile essentielle montre un effet inhibiteur très prononcé. En effet, huile essentielle, utilisée à la concentration de
0,5 %, induit une inhibition totale de la croissance des moisissures testées.
This study was undertaken to determinate the antifungal activity of essential oil from cinnamon bark. The essential oil was
tested on fungal strains using three different techniques; the agar dilution test to determine the sensitivity for the strains,
the microtitration method to determine the minimal inhibitory concentration (MIC) of the essential oil on the growth of yeasts,
and finally a macrodilution test allowing the evaluation of the essential oil on the mycelial mass production of the fungal
strains. The results obtained show that all the essential oil showed antifungal activity. The MICs were ranged from 1/640
to 1/2560 for the yeasts. For the moulds the MICs were ranged from 1/5000 to 1/10000. Evaluation of the effects of the mycelial
production showed that the essential oil had the best antifungal effect. With a concentration of 0,05% the essential oil could
totally inhibit the growth of all the species tested.
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ABSTRACT: The in vitro activity of amphotericin B, flucytosine and fluconazole against 95 yeasts causing fungemia in a single institution over the last eight years was determined by a broth macromethod recommended by the National Committee for Clinical Laboratory Standards. All strains were inhibited by amphotericin B concentrations of < or = 1 microgram/ml. With flucytosine in most species the MIC50 was between 0.12 and 0.25 microgram/ml and the MIC90 was between 0.25 and 1 microgram/ml. One exception with flucytosine was Candida krusei, with an MIC50 and MIC90 of 16 micrograms/ml and 32 micrograms/ml, respectively. Overall, 12% of the isolates needed at least 8 micrograms/ml of fluconazole to be inhibited. Fluconazole was very active against Candida albicans, Candida tropicalis and Cryptococcus neoformans, with MIC50 ranging from 0.12 to 0.5 microgram/ml and MIC90 of 1 microgram/ml, and somewhat less active against Candida parapsilosis (MIC50 of 1 microgram/ml and MIC90 of 4 micrograms/ml). Fluconazole exhibited poor in vitro activity against Candida krusei (MIC50 and MIC90 of 64 micrograms/ml) and Torulopsis glabrata (MIC50 of 4 micrograms/ml and MIC90 of 16 micrograms/ml). High MICs of fluconazole were found for four strains of Candida albicans, one with an MIC of 4 micrograms/ml and three (5.7%) with MICs of > or = 16 micrograms/ml. Previous exposure to fluconazole could be demonstrated in two of these strains. Further work must be done in order to determine appropriate breakpoints of antifungal agents, to assess the clinical relevance of azole resistance in yeasts causing bloodstream infections and to identify risk factors for infections with azole-resistant yeasts.European Journal of Clinical Microbiology 04/1995; 14(4):362-5. · 3.02 Impact Factor
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ABSTRACT: Previous work indicated that cinnamon was inhibitory to the growth of aflatoxin-producing molds. The objective of this study was to determine the specific components of cinnamon and cloves that may be effective against mold growth and toxin production. The effects of cinnamon oil, clove oil, cinnamic aldehyde and eugenol on growth and aflatoxin production by Aspergillus parasiticus were studied using yeast-extract sucrose broth as the substrate. All four substances inhibited mold growth and subsequent toxin production. Cinnamon and clove oils were inhibitory at 200–250 ppm, cinnamic aldehyde at 150 ppm and eugenol at 125 ppm. Since cinnamic aldehyde and eugenol are the respective major components of cinnamon and clove oils, it was concluded that these are the major active antifungal ingredients of these two essential oils. The inhibitory effect of these substances was judged to be inhibition of growth rather than of toxin production. When growth occurred after a delay, aflatoxin production occurred when the cultures reached secondary metabolism. Given sufficient time, cultures which were inhibited initially, but which subsequently grew, produced toxin levels equivalent to control cultures. Levels of the oils above 250 ppm and of cinnamic aldehyde and eugenol above 200 ppm completely inhibited mold growth, or permitted only a small amount of growth that never reached secondary metabolism and never produced aflatoxins during the time of this study.Journal of Food Science 06/1977; 42(4):1107 - 1109. · 1.78 Impact Factor
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ABSTRACT: Several chemically modified tetracycline analogs (CMTs), which were chemically modified to eliminate their antibacterial efficacy, were unexpectedly found to have antifungal properties. Of 10 CMTs screened in vitro, all exhibited antifungal activities, although their efficacies varied. Among these compounds, CMT-315, -3, and -308 were found to be the most potent as antifungal agents. The MICs of CMT-3 against 47 strains of fungi in vitro were determined by using amphotericin B (AMB) and doxycycline as positive and negative controls, respec- tively. The MICs of CMT-3 were generally found to be between 0.25 and 8.00 g/ml, a range that approximates the blood levels of this drug when administrated orally to humans. Of all the yeast species tested to date, Candida albicans showed the greatest sensitivity to CMT-3. The filamentous species most susceptible to CMT-3 were found to be Epidermophyton floccosum, Microsporum gypseum, Pseudallescheria boydii ,a Penicillium sp., Scedosporium apiospermum ,a Tricothecium sp., and Trichophyton rubrum. Growth inhibition of C. albicans by CMT-3, determined by a turbidity assay, indicated a 50% inhibitory concentration of 1 g/ml. Thirty-nine strains, including 20 yeasts and 19 molds, were used to measure viability (the ability to grow after treatment with a drug) inhibition by CMT-3 and AMB. CMT-3 exhibited fungicidal activity against most of these fungi, especially the filamentous fungi. Eighty-four percent (16 of 19) of the filamentous fungi tested showed more than 90% inhibition of viability by CMT-3. In contrast, AMB showed fungicidal activity against all yeasts tested. However, most of the filamentous fungi (16 of 19) showed less than 50% inhibition of viability by AMB, indicating that AMB is fungistatic against most of these filamentous fungi. To begin to identify the sites in fungal cells affected by CMT-3, C. albicans and a Penicillium sp. were incubated with the compound at 35°C, and then the fluorescence of CMT-3 was observed by confocal laser scanning electron microscopy. CMT-3 appeared to have widespread intracellular distribution throughout C. albicans and the Penicillium sp. The mechanisms of the antifungal activity of CMT-3 are now being explored. The first chemically modified tetracycline (CMT) was de- scribed in 1987 (13), soon after it was discovered that tet- racyclines (TETs) could inhibit mammal-derived matrix metalloproteinases (MMPs) in vitro and in vivo (11, 14) by mechanisms which were independent of the antibacterial efficacy of these drugs. Since then, more than 30 different CMTs, in which the 4-dimethylamino group has been de- leted, have been developed. The most prominent character-Antimicrobial Agents and Chemotherapy - ANTIMICROB AGENTS CHEMOTHER. 01/2002; 46(5):1447-1454.