Phenolic compounds and somatic embryogenesis in cotton (Gossypium hirsutum L.)

Plant Cell Tissue and Organ Culture (Impact Factor: 2.61). 01/2007; 90(1):25-29. DOI: 10.1007/s11240-007-9243-2

ABSTRACT Studies of phenolic compounds were performed during cell suspension cultures in relation with the induction of embryogenic
structures in two cultivars of cotton. Coker 312 produced embryogenic structures, unlike R405-2000 which was found to be a
non-embryogenic cultivar. Embryogenesis induction in Coker 312 was strongly linked to a higher content of caffeic, ferulic
and salicylic acids and to the appearance of p-coumaric acid, benzoic acid, trans-resveratrol, catechin and naringenin.

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    ABSTRACT: Trans-resveratrol is a phytoalexin which was found in some grapes and in many other plants. This phenolic compound has gained much attention, as it was indicated to be associated with fungus and disease resistance. Also, it has been shown that phenols compounds could play an important role in somatic embryogenesis induction of various plants. In cotton, embryogenic structures take place in cell suspension cultures. The need of biochemical indicators for the early identification of cells capable to undergo embryogenic structures formation has always been a major concern of researchers. Trans-resveratrol was found only in embryogenic cell suspensions of Coker 312. This phenolic compound started at the first subculture (2.44 µg/g dw) and the maximum level was reached at the third subculture (7.2 µg/g dw) with an increase of nearly 295%. This study showed a correlation between trans-resveratrol synthesis in cotton cell and embryogenic structures induction. trans-resveratrol may be a phenolic indicator for is induction of cotton somatic embryogenesis.
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