The influence of bakers’ yeast cells on protein adsorption in anion exchange expanded bed chromatography
ABSTRACT The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a
commercially available expanded bed column (20 mm i.d.) from Up Front Chromatography and Streamline DEAE (ρ=1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored
by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline
DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity
in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins
to bind to the sites on adsorbents.