Article

The influence of bakers’ yeast cells on protein adsorption in anion exchange expanded bed chromatography

Biotechnology and Bioprocess Engineering (impact factor: 1.28). 04/2012; 10(3):280-283. DOI:10.1007/BF02932026 pp.280-283

ABSTRACT The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a
commercially available expanded bed column (20 mm i.d.) from Up Front Chromatography and Streamline DEAE (ρ=1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored
by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline
DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity
in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins
to bind to the sites on adsorbents.

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Keywords

adsorbents
 
adsorption
 
adsorption capacity
 
Amersham Pharmacia Biotechnology
 
batch binding
 
bed chromatography
 
bed column
 
bind
 
BSA binding capacity
 
Chromatography
 
exploiting
 
Langmuir equation
 
model protein bovine serum albumin
 
proteins
 
Streamline DEAE
 
whole yeast cells
 
yeast cells
 
yeast cells act