Article

Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure

Science China-Chemistry (impact factor: 1.02). 05/2012; 54(8):1273-1276. DOI:10.1007/s11426-011-4327-6 pp.1273-1276

ABSTRACT An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method
is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is
sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present
study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes
that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes
on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical
assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.

KeywordsDNA nanostructure–surface density–electrochemical

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Keywords

branched tetrahedron structure probes
 
disfavor DNA hybridization
 
DNA capture probes
 
DNA detection
 
DNA hybridization event
 
electrochemical assay
 
electrochemical method
 
hybridization environment
 
immobilized DNA probes
 
KeywordsDNA nanostructure–surface density–electrochemical
 
regular linear single
 
sensitive
 
SNPs
 
SNPs genotyping
 
solid surfaces
 
surfaces environments
 
three-dimensional DNA nanostructure