Article

Manassantin a and b isolated fromSaururus chinensis inhibit TNF-α-induced Cell adhesion molecule expression of human umbilical vein endothelial cells

Chungnam National University College of Pharmacy Taejeon Korea
Archives of Pharmacal Research (impact factor: 1.59). 04/2012; 28(1):55-60. DOI:10.1007/BF02975136 pp.55-60

ABSTRACT Leukocyte adhesion to the vascular endothelium is a critical initiating step in inflammation and atherosclerosis. We have
herein studied the effect of manassantin A (1) and B (2), dineolignans, on interaction of THP-1 monocytic cells and human umbilical vein endothelial cells (HUVEC) and expression
of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in HUVEC. When HUVEC
were pretreated with1 and2 followed by stimulation with TNF-α, adhesion of THP-1 cells to HUVEC decreased in dose-dependent manner with IC50 values of 5 ng/mL and 7 ng/mL, respectively, without cytotoxicity. Also,1 and2 inhibited TNF-α-induced up-regulation of ICAM-1, VCAM-1 and E-selectin. The present findings suggest that1 and2 prevent monocyte adhesion to HUVEC through the inhibition of ICAM-1, VCAM-1 and E-selectin expression stimulated by TNF-α,
and may imply their usefulness for the prevention of atherosclerosis relevant to endothelial activation.

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Keywords

atherosclerosis relevant
 
critical initiating step
 
cytotoxicity
 
dose-dependent manner
 
E-selectin expression
 
herein
 
human umbilical vein endothelial cells
 
IC50 values
 
ICAM-1
 
inflammation
 
intercellular adhesion molecule-1
 
manassantin
 
present findings
 
pretreated with1 and2
 
that1 and2
 
THP-1 cells
 
THP-1 monocytic cells
 
vascular cell adhesion molecule-1
 
vascular endothelium
 
VCAM-1