High frequency plant regeneration from the mature seeds of Garcinia indica
ABSTRACT A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured
on Woody plant medium (WPM) supplemented with 2.0 mg dm−3 N6-benzyladenine and 1 mg dm−3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm−3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival
rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation
from mature seed segments.
Additional key wordsdirect organogenesis–kokum elite clones–mass multiplication–multiple shoots
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ABSTRACT: In vitro seed germination and development of Butea monosperma (Lam.) Taub. Var. lutea (Willt.) : Abstract Enhanced in vitro seed germination, seedling development protocol has been established for conservation of very rare and globally endangered woody tree species, Butea monosperma (Lam.) Taub. var. lutea (Witt.) Fabaceae). It differs from Butea monosperma var.monosperma in presence of ivory-white/ bright yellow flower buds and flowers. The plant seeds very low viability and ex vitro germination rates responsible for its rare and globally endangered status. Mature seeds were cultured on two basic inorganic media with full (F) or ½ strength (H) of MS or WPM supplemented with various concentrations of N6-benzyladenine (BA, 2. alone. Within the range evaluated, highest percentage (65%) of the seed germination (pre-hot water treated for 3 min) was obtained from seeds cultured on MSF medium containing 4.40 µM BA . The same medium has promoted the highest growth of seedling with a mean of 3.57 ± 0.06 cm shoot length and 2.44 ± 0.17 cm root length after 3 weeks of culture. BA at 6.62 µM level in WPMF medium provoked 50% seed germination response. While, TDZ (2.27µM, 0.45 µM) induced 45% and 30% response on WPMF or WPMH medium respectively. The seedlings (90%) of B. monosperma var. lutea readily acclimated to greenhouse conditions. The present investigation for the first time describes an enhanced in vitro seed germination and seedling development protocol for conservation and restoration of B. monosperma var. Lutea.