High frequency plant regeneration from the mature seeds of Garcinia indica

Biologia Plantarum (Impact Factor: 1.74). 01/2011; 55(3):554-558. DOI: 10.1007/s10535-011-0124-5

ABSTRACT A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured
on Woody plant medium (WPM) supplemented with 2.0 mg dm−3 N6-benzyladenine and 1 mg dm−3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm−3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival
rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation
from mature seed segments.

Additional key wordsdirect organogenesis–kokum elite clones–mass multiplication–multiple shoots

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