Whole genome amplification and microsatellite genotyping of herbarium DNA revealed the identity of an ancient grapevine cultivar

Naturwissenschaften (Impact Factor: 1.97). 01/2011; 98(9):763-772. DOI: 10.1007/s00114-011-0826-8

ABSTRACT Reconstruction of the grapevine cultivation history has advanced tremendously during the last decade. Identification of grapevine
cultivars by using microsatellite DNA markers has mostly become a routine. The parentage of several renowned grapevine cultivars,
like Cabernet Sauvignon and Chardonnay, has been elucidated. However, the assembly of a complete grapevine genealogy is not
yet possible because missing links might no longer be in cultivation or are even extinct. This problem could be overcome by
analyzing ancient DNA from grapevine herbarium specimens and other historical remnants of once cultivated varieties. Here,
we present the first successful genotyping of a grapevine herbarium specimen and the identification of the corresponding grapevine
cultivar. Using a set of nine grapevine microsatellite markers, in combination with a whole genome amplification procedure,
we found the 90-year-old Tribidrag herbarium specimen to display the same microsatellite profile as the popular American cultivar Zinfandel. This work, together
with information from several historical documents, provides a new clue of Zinfandel cultivation in Croatia as early as the
beginning of fifteenth century, under the native name Tribidrag. Moreover, it emphasizes substantial information potential of existing grapevine and other herbarium collections worldwide.

Vitis vinifera

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    ABSTRACT: With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status, and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However, the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen, and chemical treatment of the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols in this chapter have been successfully used for high-quality DNA extraction from different kinds of plant herbarium tissues. These methods differ primarily with respect to their requirements for input material (from algae to vascular plants), type of the plant tissue (leaves with incrustations, sclerenchyma strands, mucilaginous tissues, needles, seeds), and further possible applications (PCR-based methods or microsatellites, AFLP).
    Methods in molecular biology (Clifton, N.J.) 01/2014; 1115:69-84. · 1.29 Impact Factor