Article

Cryoprotective effects of various saccharides on cryopreserved mouse sperm from various strains

Niigata University Brain Research Institute 1-757 Asahimachi dori, Chuo-ku 951-8585 Niigata Japan
Reproductive Medicine and Biology 11/2007; 6(4):229-233. DOI: 10.1111/j.1447-0578.2007.00190.x

ABSTRACT AimCryopreservation of mouse sperm commonly uses raffinose, which is a trisaccharide, plus 3% skim milk. Because of the present
lack of knowledge of the effectiveness of any other saccharides, we examined the cryoprotective effects of various saccharides
on the viability of mouse sperm from various strains to determine which saccharides are the best cryoprotectants for mouse
sperm.

MethodsSperm from the caudae epididymides of mature C57BL/6J mice were frozen with monosaccharides (fructose, glucose, rhamnose,
xylose), disaccharides (lactose, maltose, sucrose, trehalose) or trisaccharides (melezitose, raffinose) in a range of concentrations
(4–33%). After thawing, the optimal concentration was determined to be the concentration in which there was the highest proportion
of motile sperm. In addition, sperm of inbred and hybrid mice were frozen with the saccharides at the optimal concentrations
and used for in vitro fertilization.

ResultsThe optimal concentration was 12% for the disaccharides and 18% for the trisaccharides. The fertility of all strains, except
C57BL/6J, showed the best cryoprotective effects with maltose, melezitose and raffinose when compared with fresh sperm.

ConclusionMaltose, melezitose and raffinose have the best effects when used as a protectant for cryopreservation of mouse sperm.

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