Amine functionalized TiO2–carbon nanotube composite: synthesis, characterization and application to glucose biosensing

12/2011; 1(4):189-195. DOI: 10.1007/s13204-011-0025-0

ABSTRACT The synthesis of amine functionalized TiO2-coated multiwalled carbon nanotubes (NH2-TiO2-CNTs) using sol–gel method was investigated. The synthesized nanocomposite was characterized with XRD, FTIR spectroscopy,
BET test and SEM imaging. The results demonstrated a unique nanostructure with no destruction of the CNTs’ shape. In addition,
the presence of amine groups on the composite surface was confirmed by FTIR. This nanocomposite was used for one-step immobilization
of glucose oxidase (GOx) to sense glucose. The result of cyclic voltammetry showed a pair of well-defined and quasi-reversible
peaks for direct electron transfer of GOx in the absence of glucose. Also, the result of electrochemical impedance spectroscopy
indicated that GOx was successfully immobilized on the surface of NH2-TiO2-CNTs. Furthermore, good amperometric response showed that immobilized GOx on the NH2-TiO2-CNTs exhibits exceptional bioelectrocatalytic activity toward glucose oxidation.

KeywordsCarbon nanotube–TiO2
–Glucose oxidase–Bioelectrocatalysis

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