Article

Simultaneous Determination of Four Sudan Dyes in Egg Yolks by Molecularly Imprinted SPE Coupled with LC-UV Detection

Chromatographia (impact factor: 1.2). 04/2012; 73(3):227-233. DOI:10.1007/s10337-010-1872-7 pp.227-233

ABSTRACT A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection
was developed for the simultaneous isolation and determination of four Sudan dyes (I, II, III and IV) in egg-yolk products.
The imprinted microspheres synthesized by suspension polymerization using phenylamine–naphthol as mimic template show high
selectivity and affinity to the four kinds of Sudan dyes and were successfully applied as selective sorbents of solid-phase
extraction for the simultaneous determination of the four Sudans from egg-yolk samples. Good linearity was obtained in a range
of 0.062–10μgg−1 and the average recoveries of the four Sudans at three spiked levels ranged from 94.1 to 102.5% with the relative standard
deviations less than 5.8%. The developed extraction protocol eliminated the effect of template leakage on quantitative analysis
and could be applied for the determination of Sudans in complicated food samples.

KeywordsColumn liquid chromatography–Molecularly imprinted solid-phase extraction–Imprinted microspheres–Sudan dyes–Egg yolk

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Keywords

affinity
 
average recoveries
 
developed extraction protocol
 
egg-yolk products
 
egg-yolk samples
 
four kinds
 
four Sudans
 
Good linearity
 
imprinted microspheres synthesized
 
KeywordsColumn liquid chromatography–Molecularly imprinted solid-phase extraction–Imprinted microspheres–Sudan dyes–Egg yolk
 
MISPE
 
relative standard
 
selective molecularly imprinted solid-phase extraction
 
simultaneous isolation
 
spiked levels
 
Sudan dyes
 
Sudans
 
suspension polymerization
 
template leakage