Determination of Tannic Acid by Flow Injection Analysis with Inhibited Chemiluminescence Detection

Microchimica Acta (Impact Factor: 3.72). 09/2006; 155(3):427-430. DOI: 10.1007/s00604-006-0628-4

ABSTRACT A novel flow injection procedure has been developed for the determination of tannic acid based on the inhibition of the chemiluminescences
in luminol-H2O2-Manganese tetrasulfonatophthalocyanine (MnTSPc) system by tannic acid. The method is simple, rapid and sensitive with a detection
limit of 8 × 10−10 mol·L−1 and a linear range of 7 × 10−9–5 × 10−6 mol·L−1. The relative standard deviation is 1.9% for eleven measurements of 5 × 10−7 mol·L−1 tannic acid. The method has been successfully applied to the determination of tannic acid in real Chinese gall and hop pellets

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    ABSTRACT: A highly sensitive flow-injection chemiluminescent method for the direct determination of pyrogallol compounds has been developed. Proposed method is based on the enhanced effect of pyrogallol compounds on the chemiluminescence signals of KMnO(4)-H(2)O(2) system in slightly alkaline medium. Three important pyrogallol compounds, pyrogallic acid, gallic acid and tannic acid, have been detected by this method, and the possible mechanism of the CL reaction is also discussed. The proposed method is simple, convenient, rapid (60 samples h(-1)), and sensitive, has a linear range of 8x10(-10) mol L(-1) to 1x10(-5) mol L(-1), for pyrogallic acid, with a detection limit of 6x10(-11) mol L(-1), 4x10(-8) mol L(-1) to 5x10(-3) mol L(-1) for gallic acid with a detection limit of 9x10(-10) mol L(-1), and 8x10(-8) mol L(-1) to 5x10(-2) mol L(-1) for tannic acid, with a detection limit of 2x10(-9) mol L(-1), respectively. The relative standard deviation (RSD, n=15) was 0.8, 1.1 and 1.3% for 5x10(-6) mol L(-1) pyrogallic acid, gallic acid and tannic acid, respectively. The proposed method was successfully applied to the determination of pyrogallol compounds in tea and coffee samples.
    Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 08/2009; 74(5):1046-9. DOI:10.1016/j.saa.2009.08.047 · 2.13 Impact Factor
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    ABSTRACT: A flow system, coupled with visible light scattering method, was developed for tannin determination based on the ability of the tannic acid to precipitate bovine serum albumin (BSA). The amount of precipitated tannic acid- BSA is measured directly by scattering intensity of visible light (600 nm), which is properly related to the tannic acid concentration and conformed by a particle sizing method. Scattering signals were non-significant compare with background produced by BSA in both solutions lower than pH4 and higher than pH8. The tannic acid-protein precipitates on pipeline wall and sensing cell could be effectively removed by 22 cm of SDS stream. The system performed a linear detection range 70-100 μg/ml of tannic acid with a detect limit 40 μg/ml in pH5 solution. It also could be used in medium solution with detection ranges were over 420-500 μg/ml and 700-1000 μg/ml in pH6 and pH 7, respectively. The proposed method had good correlations (r2 > 0.97) with the ferrous tartrate method in analysis of both one tannic acid and two tea tannins samples. Keywordstannic acid–precipitation–light scattering
    01/2010: pages 237-240;
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    ABSTRACT: Tannic acid can form a precipitate with bovine serum albumin (BSA), and this was explored for its determination using a flow injection system along with detection via light scattering at 600nm. The results on light scattering are confirmed using a particle sizing method. Scattering signals are negligible compared to the background generated by BSA in solutions with a pH of <4 or >8. Any precipitates on the wall of the flow cell pipeline were effectively removed by using a sodium dodecyl sulfate washing solution. The analytical range is from 70 to 250µgmL-1 of tannic acid. The results imply that increasing BSA concentrations will enhance sensitivity and analytical ranges. The system can be used in solutions of pH values between 5 and 7. The results correlated closely (r2 > 0.97) with those obtained by the ferrous tartrate method when analyzing a single tannic acid and two tea tannins. KeywordsTannic acid-Bovine serum albumin (BSA)-Precipitation-Light scattering
    Microchimica Acta 04/2010; 169(1):117-122. DOI:10.1007/s00604-010-0301-9 · 3.72 Impact Factor
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