Article

Selective determination of uric acid in the presence of ascorbic acid at poly(p-aminobenzene sulfonic acid)-modified glassy carbon electrode

People’s Hospital of Gansu Province Lanzhou 730000 People’s Republic of China; Chinese Academy of Sciences Graduate School Beijing 100039 People’s Republic of China
Journal of Applied Electrochemistry (impact factor: 1.75). 04/2012; 39(12):2323-2328. DOI:10.1007/s10800-009-9916-0 pp.2323-2328

ABSTRACT The electrocatalytic behavior of uric acid has been investigated with a glassy carbon electrode modified with p-aminobenzene sulfonic acid through electrochemical polymerization. This resulting electrode shows an excellent electrocatalytic
response to uric acid and ascorbic acid, with a peak-to-peak separation of 0.267V in a 0.1molL−1 phosphate buffer solution (PBS) at pH 7.0. These results indicate that the proposed electrode can eliminate the serious interference
of ascorbic acid, which coexists with uric acid in body fluids. Differential pulse voltammetry (DPV) was used for detecting
uric acid with selectivity and sensitivity. The anodic peak current of uric acid was proportional to its concentration in
the range of 1.2×10−7–8.0×10−4molL−1, with a detection limit of 4.0×10−8molL−1. The proposed method has been applied with satisfactory results to the determination of uric acid in human urine without
any pretreatment.

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Keywords

0.1molL−1 phosphate buffer solution
 
ascorbic acid
 
body fluids
 
detection limit
 
Differential pulse voltammetry
 
electrocatalytic behavior
 
electrochemical polymerization
 
glassy carbon electrode
 
human urine
 
p-aminobenzene sulfonic acid
 
peak-to-peak separation
 
pretreatment
 
proposed electrode
 
proposed method
 
satisfactory results
 
uric acid
 

Hao Tang