Article

Enantiomeric Separation of Nefopam Hydrochloride by Affinity Electrokinetic Chromatography Using Chondroitin Sulfate A as Chiral Selector and Its Chiral Recognition Mechanism

Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009 China
Chromatographia (Impact Factor: 1.37). 09/2010; 72(5):489-493. DOI: 10.1365/s10337-010-1670-2

ABSTRACT In this study, a new approach to the enantioseparation of nefopam hydrochloride by means of affinity electrokinetic chromatography
(AEKC) with chondroitin sulfate A belonging to linear ionic polysaccharides has been developed. The difference in the antinociceptive
activity of the enantiomers of nefopam was demonstrated in some studies, and the method established in this paper allowed
complete separation of nefopam. Especially, there are no reports concerned with the enantioselective separation of nefopam
using chondroitin sulfate A as chiral selectors in CE. During the course of this work, both migration time and enantioseparation
of nefopam were influenced by several parameters such as pH of the BGE, selector concentration, capillary temperature and
applied voltage. Consequently, these parameters were systematically optimized in order to obtain the optimum enantioseparation
of nefopam. Moreover, comparison of the influences of the studied parameters was further investigated using univariate analysis
of variance as a calculation method by Statistical Product and Service Solutions (SPSS) in this paper. Finally, a mechanism
of enantiorecognition in AEKC towards the enantiomers of nefopam with chondroitin sulfate A was described.

KeywordsAffinity electrokinetic chromatography-Enantiomeric separation-Polysaccharides-Nefopam hydrochloride-Chondroitin sulfate A

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