Article

Study of the Essential Oil Composition of Cumin Seeds by an Amino Ethyl-Functionalized Nanoporous SPME Fiber

Chromatographia (Impact Factor: 1.37). 10/2009; 70(7):1147-1151. DOI: 10.1365/s10337-009-1269-7

ABSTRACT An ultrasound assisted SPME method with a new nanoporous SBA-15 fiber functionalized with 3-[bis(2-hydroxyethyl)amino]propyl-triethoxysilane
was successfully applied to the study of the essential oil composition of cumin (Cuminum cyminum L.) seeds. The sample was irradiated by ultrasound radiation and its volatile components were collected by the fiber from
the sample headspace and directly injected into a GC-MS injection port for analysis. A simplex method was used for optimization
of four different parameters affecting the efficiency of the extraction. Under the optimized conditions (i.e. sample weight,
0.6g; temperature, 70°C; sonication time, 12min and extraction time, 28min), the number of components identified by the
proposed method and their amounts were identical to those of a hydrodistillation technique. The extraction efficiency of the
SBA-15 fiber was superior to a PDMS commercial fiber. The major components identified were p-menta-1,3-dien-7-al, cuminaldehyde, γ-terpinene and p-cymene, respectively. The proposed method was applied to a comparative study of the essential oil composition of cumin in
three different climate conditions of the Lorestan province in Iran. The results indicated that the essential oils in the
temperate and tropical locations were 94.0 and 85.6% of the cold region, respectively.

Download full-text

Full-text

Available from: Payman Hashemi, Jul 06, 2015
0 Followers
 · 
381 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: INTRODUCTION: The development of simple, rapid and solvent-free methods for the analysis of essential oils is highly desirable. Microwave-assisted headspace solid-phase microextraction (MA-HS-SPME) is a new sampling and concentration technique for the extraction of volatile components in medicinal plants. The main advantages of this method are the reduction of extraction time and of organic solvent. OBJECTIVE: A highly porous Santa Barbara amorphous (SBA-15)/polyaniline material was prepared in order to produce a SPME fibre. The proposed fibre was evaluated for the extraction of the volatile component of Teucrium polium L. METHODOLOGY: A homemade MA-HS-SPME apparatus was used for the extraction of volatile components. Highly porous SBA-15/polyaniline materials were prepared for SPME. The prepared nanomaterial was immobilized onto a stainless steel wire for fabrication of the SPME fibre. RESULTS: The SBA-15/polyaniline nanonporous fibre could adsorb volatile components of T. polium efficiently. In comparison with a HD method, the proposed technique could equally monitor almost all the components of the sample, but in an easier way that was rapid and required a much lower amount of sample. CONCLUSION: The experimental results showed that the nanoporous fibre was suitable for the semi-quantitative study of the composition of essential oils in plant materials and monitoring the variations in the volatile components of the plants. Copyright © 2012 John Wiley & Sons, Ltd.
    Phytochemical Analysis 01/2013; 24(1). DOI:10.1002/pca.2382 · 2.45 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully used in isolation and purification of cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by using a two-phase solvent system composed of n-hexane-methanol-water (5:4:1, v/v/v). The targeted compounds were isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by gas chromatography (GC). A total of 12.72 ± 0.22 mg of cuminaldehyde and 10.61 ± 0.27 mg of p-menta-1,4-dien-7-al were obtained from 50 mg of the essential oil of C. cyminum L. in less than 6 h, with purities of 95.42% and 97.21%, respectively. In addition to GC-EI/MS, the identity of the cuminaldehyde was further confirmed with the retention time using the method of standard addition, while, the structural identification of p-menta-1,4-dien-7-al was performed with GC-EI/MS, (1)H NMR and (1)H-(1)H COSY.
    Analytica chimica acta 03/2011; 689(1):149-54. DOI:10.1016/j.aca.2011.01.038 · 4.52 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cuminum cyminum and Carum carvi are the sources of cumin and caraway seeds respectively, which have been used since antiquity for the treatment of various indications in traditional healing systems in wide geographical areas. Cumin and caraway seeds are rich sources of essential oils and have been actively researched for their chemical composition and biological activities. In recent times (especially during the last 3 years) considerable progress has been made regarding validation of their acclaimed medicinal attributes by extensive experimental studies. In this attempt many novel bioactivities have been revealed. This review highlights the significance of cumin and caraway as potential source of diverse natural products and their medicinal applications.
    Pharmacognosy Reviews 03/2011; 5(9):63-72. DOI:10.4103/0973-7847.79101