Article

Sugarcane Phosphoribosyl Pyrophosphate Synthetase: Molecular Characterization of a Phosphate-independent PRS

Universidade Estadual de Goiás Ciências Exatas e Tecnológicas BR 153 Km 18 75001-970 Anápolis GO Brazil
Plant Molecular Biology Reporter (impact factor: 2.45). 04/2012; 26(4):301-315. DOI:10.1007/s11105-008-0043-6 pp.301-315

ABSTRACT Phosphoribosyl pyrophosphate synthetase (PRS—EC:2.7.6.1) is an important enzyme present in several metabolic pathways, thus
forming a complex family of isoenzymes. However, plant PRS enzymes have not been extensively investigated. In this study,
a sugarcane prs gene has been characterized from the Sugar Cane Expressed Sequence Tag Genome Project. This gene contains a 984-bp open reading
frame encoding a 328-amino acid protein. The predicted amino acid sequence has 77% and 78% amino acid sequence identity to
Arabidopsis thaliana and Spinacia oleracea PRS4, respectively. The assignment of sugarcane PRS as a phosphate-independent PRS isoenzyme (Class II PRS) is verified following
enzyme assay and phylogenetic reconstruction of PRS homologues. To gain further insight into the structural framework of the
phosphate independence of sugarcane PRS, a molecular model is described. This model reveals the formation of two conserved
domains elucidating the structural features involved in sugarcane PRS phosphate independence. The recombinant PRS retains
secondary structure elements and a quaternary arrangement consistent with known PRS homologues, based on circular dichroism
measurements.

0 0
 · 
0 Bookmarks
 · 
27 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

328-amino acid protein
 
78% amino acid sequence identity
 
984-bp open reading
 
Class II PRS
 
metabolic pathways
 
phosphate independence
 
phosphate-independent PRS isoenzyme
 
Phosphoribosyl pyrophosphate synthetase
 
plant PRS enzymes
 
predicted amino acid sequence
 
PRS homologues
 
recombinant PRS
 
secondary structure elements
 
Spinacia oleracea PRS4
 
structural features
 
structural framework
 
Sugar Cane Expressed Sequence Tag Genome Project
 
sugarcane PRS
 
sugarcane prs gene
 
sugarcane PRS phosphate independence