Temporary immersion system in plant micropropagation. Plant Cell Tiss Org Cult

Centre de Coopération Internationale en Recherche Agronomique pour le Développement – Amis (CIRAD-AMIS), CIRAD
Plant Cell Tissue and Organ Culture (Impact Factor: 2.13). 05/2002; 69(3):215-231. DOI: 10.1023/A:1015668610465

ABSTRACT Temporary immersion systems for plant micropropagation have been described and grouped into 4 categories according to operation: tilting and rocker machines; complete immersion of plant material and renewal of the nutrient medium; partial immersion and a liquid nutrient renewal mechanism; complete immersion by pneumatic driven transfer of liquid medium and without nutrient medium renewal. The positive effects of temporary immersion on micropropagation are indicated for shoot proliferation and microcuttings, microtuberization and somatic embryogenesis. Immersion time, i.e. duration or frequency, is the most decisive parameter for system efficiency. Optimizing the volume of nutrient medium and the volume of the culture container also substantially improves efficacy, especially for shoot proliferation. Temporary immersion also generally improves plant material quality. It results in increased shoot vigour and in the frequency of morphologically normal somatic embryos. Hyperhydricity, which seriously affects cultures in liquid medium, can be eliminated with these culture systems or controlled by adjusting the immersion times. Plant material propagated by temporary immersion can perform better during the acclimatization phase than material obtained on semi-solid or in liquid media. Successful regeneration of plants, after direct sowing on soil of Solanum tuberosum microtubers and Coffea arabica somatic embryos produced in temporary immersion bioreactors, has been demonstrated. As could be expected when using liquid medium for micropropagation, several estimations confirm large gains in efficacy from temporary immersion. The parameters most involved in reducing production costs include: (1) the drastic reduction in work; (2) reduction in shelving area; (3) reduction in the number of containers used; (4) better biological yields. Scaling-up somatic embryogenesis and shoot proliferation procedures involving temporary immersion systems in order to commercialize this process are now taking place.

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Available from: hervé Etienne, Sep 27, 2015
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    • "On the other hand in a temporary immersion system, Jiménez et al. (1999) observed that the best growth of potato shoots at an immersion frequency of 5 min for every 3 h. Etienne and Berthouly (2002) reported that 1 h of immersion per 6 h interval was useful for microtuberization of potato. In our NSB system, a nutrient spray frequency of once per hour was optimum for shoot growth and microtuberization. "
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    ABSTRACT: A laboratory scale bioreactor system has been developed using nutrient spray technology for in vitro mass production of potato microtubers. Its effectiveness on the production of microtubers was investigated and compared with conventional liquid and semi-solid culture systems through bioreactor. Optimal culture conditions such as spray intervals, varying concentrations of 6-benzylaminopurine (BAP) and explants density were determined for the NSB. In order to determine optimal spray intervals, liquid medium was sprayed inside the NSB at different intervals (½, 1–4 h) of which the 1 h interval resulted in the highest number of shoots (3.47) and length (8.99 cm). Number of microtubers produced (5.13) was highest with 1 h intervals and fresh weight of microtubers (0.90 g) was highest for ½ h interval. Different concentrations of BAP (0.5, 1.0 and 1.5 mg/L) were used to evaluate its effect on microtuberization. It was observed that number and diameter of microtubers were increased (5.31 and 0.96 cm) when 0.5 mg/L BAP was supplemented in MS medium. We found fresh weight of microtubers (0.97 g) was increased when 1.0 mg/L BAP were added to the medium. In order to determine suitable explants density, single nodes grouped into five categories e.g 30, 45, 60, 75, and 90 and placed in the NSB system. A density of 60 explants resulted in increases in shoot length (17.5 cm) number of internodes (12.5) and with highest amount of chlorophyll (40.2 mg/g) as well as with highest number and fresh weight of microtubers (4.43 and 0.89 g, respectively). Out of the three culture systems, the NSB performed best where 1.5–2.0 fold increases in shoot growth and microtuberization without hyperhydration. The NSB also produced the highest number (4.67), fresh weight (0.86 g) and diameter (0.78 cm) of microtubers. From this study we may conclude that the NSB system has good potential for commercial mass production of potato micro-tuber.
    Scientia Horticulturae 08/2015; 192:369-374. DOI:10.1016/j.scienta.2015.06.014 · 1.37 Impact Factor
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    • "Offshoot propagation is a slow method and the mortality of offshoot is usually high [5]. Therefore, the use of plant tissue culture technique for propagation of date palmis considered an alternative method to the conventional methods [36].However, the extensive use of plant tissue culture technique has been limited by the high costs of media [6].The use of semisolid medium for commercial production is still hampered by low plantlet production rates, high labor cost and more space requirement [7].Therefore, the appropriate choice of media components should be considered for the production of cost effective and valued in vitro plantlets.. Liquid media have been used as an efficient method for mass propagation facilitating automation and a reduction in cost and time [1] [11]. The advantages of liquid culture systems are: uniform culture conditions, easy media replacement without changing the container, sterilization with ultra-filtration and easier container cleaning after use. "
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    ABSTRACT: The most common methods of micropropagationof date palm involve the proliferation of shoots via a semi solidsystem. While such semi solid systems have been moderately to highly successful in terms ofmultiplication yields, it istypically a labor intensive time taking means of clonal propagation. , Therefore, it is necessary to find cheaper alternatives without compromising on the quality of in vitrogrowth. This research focuses on the comparison between the liquid culture and semi-solid culture on the performance of three popular date palm cultivars i.e.Sukry, Medjool and Reziz. The results of multiplication stage indicated that liquid culturehave a significant effect on the buds number, whereas semi-solid culture have a significant effect on shoots growth. Rooting on semi-solid culture have a significant effect on roots growth while liquid culturewas significantly increased fresh and dry weights.. It appears more economical to use liquid culturethan semi-solid culture for in vitro propagation of date palm. However, liquid culture system should be employed on large-scale experiments before application for commercial production.
    Advances in Environmental Biology 12/2014; 8(16):263-269.
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    • "TIS, among other advantages, allows for the semiautomation of the culture process, facilitates medium renewal, and also combines aeration and explant immersion with programmed duration and frequency of immersion, thus reducing hyperhydricity of the plant and increasing survival during the acclimatization stage (Etienne and Berthouly 2002; Levin and Tanny 2004). The RITA ® (Récipient à Immersion Temporaire Automatique) system has been used in the micropropagation of several species including Malus sp. "
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    ABSTRACT: Here, we evaluated the efficiency of shoot multiplication of Vanilla planifolia Jacks. ex Andrews using solid medium, partial immersion, and a temporary immersion system (TIS) to improve micropropagation in this species. Clusters of shoots were cultivated in vitro using Murashige and Skoog (MS) medium supplemented with 9.55 μM benzyladenine (BA) and 100 mL L−1 coconut water. For the TIS, a RITA® system was used and three immersion frequencies were evaluated (every 4, 8, and 12 h) with an immersion time of 2 min. After 30-d culture, the TIS produced the maximum multiplication rate (14.27 shoots per explant) when using an immersion frequency of 2 min every 4 h, followed by the partial immersion system (8.64 shoots per explant), and solid medium (5.80 shoots per explant). Next, the effect of the volume of culture medium per explant was also evaluated for TIS. The most suitable volume of culture medium for shoot formation was 25 mL per explant, which increased the rate of multiplication to 17.54 shoots per explant. Root initiation was 90% successful in TIS using half-strength MS medium supplemented with 0.44 μM naphthaleneacetic acid (NAA) and an immersion frequency of 2 min every 4 h. With this system, the shoot multiplication rate increased threefold compared to that obtained with solid medium. In addition, this system produced good results for the transplantation and acclimation (90% of survival) of in vitro-derived plants. These results offer new options for large-scale micropropagation of vanilla.
    In Vitro Cellular & Developmental Biology - Plant 10/2014; 50(5). DOI:10.1007/s11627-014-9602-8 · 0.98 Impact Factor
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