Species identification and phylogenetic analysis of genus Nassarius (Nassariidae) based on mitochondrial genes

Chinese Journal of Oceanology and Limnology (Impact Factor: 0.66). 05/2010; 28(3):565-572. DOI: 10.1007/s00343-010-9031-4


Genus Nassarius contains many subgenera, such as Zeuxis, Telasco, Niotha, Varicinassa, Plicarcularia, Nassarius s. str. and Reticunassa. On the basis of morphological characteristics of the shell and radula and sequences of mitochondrial cytochrome oxidase
subunit I (COI) and 16S rRNA genes, Nassarius specimens collected from the South China Sea were identified and phylogenetically analyzed. Although Nassarius sp. and Nassarius (Varicinassa) variciferus were morphologically different in their shells, few variations were found among their radular teeth and sequences of mtCOI
and mt16S RNA genes. Therefore, Nassarius sp. should be classified as N. (Varicinassa) variciferus. Nassarius (Zeuxis) sp. has only a subtle difference from Nassarius (Zeuxis) algidus on the shell, but it shows obvious differences in radular teeth and DNA sequence, indicating that they are two distinct species.
Sequence divergence of mtCOI and mt16S RNA genes within Nassarius species was much lower than that between species, suggesting that these two genes are suitable for Nassarius species identification. Phylogenetic analysis (neighbor-joining and maximum parsimony) based on mtCOI and mt16S rRNA genes
revealed the presence of two groups in genus Nassarius and a closest relationship between subgenera Zeuxis and Telasco. Species of subgenus Plicarcularia did not form a single clade. The molecular phylogeny was not congruent with the previous morphological phylogeny. The subgeneric
divisions of genus Nassarius appear to be uncertain and unreliable.

-species identification-mtCOI gene-mt16S rRNA gene

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    • "Unfortunately, until now there are few large-scale reliable genetic studies to identify Nassarius species and estimate the level of cryptic diversity within Nassarius. Li et al. [38] employed mitochondrial sequences to study the identification and phylogeny of Nassarius. Nevertheless, due to the very limited samples, the status of Nassarius species is still unclear. "
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