The enhancement of osteogenesis by scaffold based on mineralized recombinant human-like collagen loading with rhBMP-2

Journal of Wuhan University of Technology-Mater Sci Ed (Impact Factor: 0.48). 24(6):956-960. DOI: 10.1007/s11595-009-6956-6

ABSTRACT A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly(lactic
acid) (nHA/RHLC/PLA), was prepared with recombinant human bone morphogenic protein-2 (rhBMP-2) for improving the osteoinductive property
of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 µg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model.
The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at
4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded
with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property
of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.

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