Article

Silencing of UBP43 by shRNA enhances the antiviral activity of interferon against hepatitis B virus

Virologica Sinica 01/2008; 23(5):339-344. DOI: 10.1007/s12250-008-2960-9

ABSTRACT Previous studies have shown that expression of the interferon-sensitive gene (ISG)15 protease UBP43 is increased in the liver
biopsy specimens of patients who do not respond to interferon (IFN)-α therapy. We hypothesized that UBP43 might hinder the
ability of IFN to inhibit HBV replication. In this study, we investigated whether vector-based siRNA promoted by H1 (psiUBP43)
could enhance IFN inhibiting HBV replication in cell culture. UBP43 was specifically silenced using shRNA. In HepG2.2.15 cells,
the HBeAg and HBV DNA levels were significantly reduced by IFN after transfection of shRNA, imphicated that vector-based siRNA
promoted by H1 (psiUBP43) could enhance IFN inhibiting HBV replication in cell culture. These data suggest that UBP43 modulates
the anti-HBV type I IFN response, and is a possible therapeutic target for the treatment of HBV infection.

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