Bioanalysis of Cepharanthine by LC–ESI–MS–MS and Its Application to Pharmacokinetic Studies

Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023 China; Graduate School of Chinese Academy of Sciences, Beijing, 100049 China
Chromatographia (Impact Factor: 1.44). 01/2011; 73(1):75-81. DOI: 10.1007/s10337-010-1823-3

ABSTRACT A sensitive liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS–MS) method was developed and
validated for the quantification of cepharanthine (CEP) in beagle dog plasma. The chromatographic separation was performed
on an Agilent-C18 column and the mobile phase was composed of methanol:water with 10mM ammonium acetate (20:80, v/v). Detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring
mode (MRM) to monitor m/z transitions 607→365 for CEP and 285→193 for the internal standard (IS) diazepam. This method exhibited a linear range
of 5–2,500ngmL−1. The precision (RSD%) and accuracy (RME%) of the assay were <8.7 and 2.4%, respectively. The limit of quantification was
5ngmL−1 and no significant matrix effect was observed. The validated method has been successfully applied to pharmacokinetic study
of CEP in beagle dog.

KeywordsColumn liquid chromatography–tandem mass spectrometry–Pharmacokinetic study–Cepharanthine