Article

Protective effects of immunophilin ligands on testicular torsion/detorsion damage in rats

Tehran University of Medical Sciences Basic Medical Sciences Research Center, Imam Khomeini Hospital Tehran Iran
International Urology and Nephrology (Impact Factor: 1.29). 01/2009; 41(1):93-99. DOI: 10.1007/s11255-008-9453-5

ABSTRACT ObjectivesThe purpose was to investigate the role of immunophilin ligands in ischemia/reperfusion (I/R)-induced germ cell apoptosis
in the rat.

Materials and methodsSprague–Dawley rats were divided into five groups with ten animals in each. In animals undergoing torsion/detorsion, right
testes were rotated 720º for 1h. A baseline group was for basal normal values. The sham-operated group served as a control
group. The TD group underwent torsion/detorsion surgery alone; the cyclosporine-A group (TD-CsA) received intravenous cyclosporine
injection (5mg/kg) at the time of detorsion, and the FK-506 group (TD-FK) received intravenous FK-506 (3.5mg/kg) at the
time of detorsion. For measurement of lipid peroxidation and antioxidant enzyme activities, the right testes of five animals
in each group were excised after 4-h reperfusion. Germ cell apoptosis indices were determined 24h following detorsion in
the right testes of the remaining five animals in each group.

ResultsMalondialdehyde (MDA) levels in the TD group were significantly higher compared to control and baseline groups. Moreover,
testicular MDA values in TD-CsA and TD-FK groups were significantly lower than in TD. There were also significant decreases
in catalase and superxide dismutase activities in the TD group compared to control and baseline groups. These values in TD-CsA
and TD-FK groups were significantly higher than in TD. The mean germ cell apoptosis scores were significantly higher in TD
animals compared to control and baseline groups; however, CsA and FK-506 treatment significantly reduced the apoptosis compared
with the TD group.

ConclusionWe have shown that administration of immunophilin ligands in testicular torsion decreases ischemia/reperfusion (I/R) cellular
damage. The results of biochemical studies suggest that reduction of oxidative stress along with attenuated neutrophil accumulation
by immunophilin ligands may have a major role in their cytoprotective effects.

Full-text

Available from: Reza Rahimian, Apr 26, 2015
1 Follower
 · 
230 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the expression of Cold-inducible RNA-binding protein (CIRP) in torsion/detorsion of the testes in different phases and demonstrate the protective effect of CIRP on testicular injury after torsion/detorsion (T/D) in an experimental mouse model. Twenty-four male BALB/c mice were divided randomly into 8 groups: normal control group (N), sham-operated group (S), torsion 2h group (T2h), torsion/detorsion 12h group (T/D12h), and T/D24h, T/D48h, T/D72h, and T/D96h groups. The testes were examined for the expression levels of CIRP. Another 32 male BALB/c mice were divided randomly in to 4 groups: normal control group (N), T/D group, T/D+pcDNA3.1 group, and T/D+pcDNA3.1-CIRP group. The plasmids were transfected into testes with in vivo-jetPEI. After 3days, morphological changes, mean seminiferous tubule diameter (MSTD), and the number of the germ cell layers were observed. Superoxide dismutase (SOD) activity, the levels of malondialdehyde (MDA), and Bcl-2/Bax ratios were studied in the different groups. Compared with the N and S groups, the expression of CIRP in the T2h group was down-regulated. In T/D groups, the levels of CIRP were reduced in a time dependent manner. Compared to T/D and T/D+pcDNA3.1 group, the MSTD, number of the germ cell layers, SOD activity, and Bcl-2/Bax ratio increased in T/D+pcDNA3.1-CIRP group, while the level of MDA decreased. The results of our study have shown that down-regulated CIRP is involved in testicular injury after testicular torsion/detorsion. Up-regulation of the expression of CIRP may reduce the damage caused by torsion/detorsion, possibly by preventing germ cell oxidative stress and apoptosis.
    Journal of Pediatric Surgery 10/2013; 48(10):2140-2147. DOI:10.1016/j.jpedsurg.2013.02.065 · 1.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Testicular torsion is one of the few emergencies in pediatric urology which requires an accurate and timely diagnosis in order to avoid testis loss. It is not an uncommon event affecting a young male population. In fact, testicular torsion is more common than testicular tumors for this same age group, yet testicular torsion has not been given the public attention it deserves as a male health risk. In this review we highlight the new information published over the past four years regarding testicular torsion. We will discuss a variety of topics associated with torsion including: medical legal issues, etiology and genetics, imaging diagnostics, innovative surgical techniques, management controversies, fertility, and new drug therapies.
    Journal of pediatric urology 10/2012; 9(6). DOI:10.1016/j.jpurol.2012.08.012 · 1.41 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mammalian spermatogenesis is a complex biological process occurring in the seminiferous tubules in the testis. This process represents a delicate balance between cell proliferation, differentiation, and apoptosis. In most mammals, the testicles are kept in the scrotum 2 to 7°C below body core temperature, and the spermatogenic process proceeds with a blood and oxygen supply that is fairly independent of changes in other vascular beds in the body. Despite this apparently well-controlled local environment, pathologies such as varicocele or testicular torsion and environmental exposure to low oxygen (hypoxia) can result in changes in blood flow, nutrients, and oxygen supply along with an increased local temperature that may induce adverse effects on Leydig cell function and spermatogenesis. These conditions may lead to male subfertility or infertility. Our literature analyses and our own results suggest that conditions such as germ cell apoptosis and DNA damage are common features in hypoxia and varicocele and testicular torsion. Furthermore, oxidative damage seems to be present in these conditions during the initiation stages of germ cell damage and apoptosis. Other mechanisms like membrane-bound metalloproteinases and phospholipase A2 activation could also be part of the pathophysiological consequences of testicular hypoxia.
    Oxidative Medicine and Cellular Longevity 09/2012; 2012:929285. DOI:10.1155/2012/929285 · 3.36 Impact Factor