Protective effects of immunophilin ligands on testicular torsion/detorsion damage in rats

Tehran University of Medical Sciences Basic Medical Sciences Research Center, Imam Khomeini Hospital Tehran Iran
International Urology and Nephrology (Impact Factor: 1.52). 03/2009; 41(1):93-99. DOI: 10.1007/s11255-008-9453-5

ABSTRACT ObjectivesThe purpose was to investigate the role of immunophilin ligands in ischemia/reperfusion (I/R)-induced germ cell apoptosis
in the rat.

Materials and methodsSprague–Dawley rats were divided into five groups with ten animals in each. In animals undergoing torsion/detorsion, right
testes were rotated 720º for 1h. A baseline group was for basal normal values. The sham-operated group served as a control
group. The TD group underwent torsion/detorsion surgery alone; the cyclosporine-A group (TD-CsA) received intravenous cyclosporine
injection (5mg/kg) at the time of detorsion, and the FK-506 group (TD-FK) received intravenous FK-506 (3.5mg/kg) at the
time of detorsion. For measurement of lipid peroxidation and antioxidant enzyme activities, the right testes of five animals
in each group were excised after 4-h reperfusion. Germ cell apoptosis indices were determined 24h following detorsion in
the right testes of the remaining five animals in each group.

ResultsMalondialdehyde (MDA) levels in the TD group were significantly higher compared to control and baseline groups. Moreover,
testicular MDA values in TD-CsA and TD-FK groups were significantly lower than in TD. There were also significant decreases
in catalase and superxide dismutase activities in the TD group compared to control and baseline groups. These values in TD-CsA
and TD-FK groups were significantly higher than in TD. The mean germ cell apoptosis scores were significantly higher in TD
animals compared to control and baseline groups; however, CsA and FK-506 treatment significantly reduced the apoptosis compared
with the TD group.

ConclusionWe have shown that administration of immunophilin ligands in testicular torsion decreases ischemia/reperfusion (I/R) cellular
damage. The results of biochemical studies suggest that reduction of oxidative stress along with attenuated neutrophil accumulation
by immunophilin ligands may have a major role in their cytoprotective effects.

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