Article

Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

Clinical Oral Investigations (impact factor: 2.36). 04/2012; 13(4):383-391. DOI:10.1007/s00784-009-0260-x pp.383-391

ABSTRACT Recently, osteogenic precursor cells were isolated from human dental follicles, which differentiate into cementoblast- or
osteoblast-like cells under in vitro conditions after the induction with dexamethasone or insulin. However, mechanisms for
osteogenic differentiation are not understood in detail. In a previous study, real-time RT-PCR results demonstrated molecular
mechanisms in dental follicle cells (DFCs) during osteogenic differentiation that are different from those in bone-marrow-derived
mesenchymal stem cells. We analysed gene expression profiles in DFCs before and after osteogenic differentiation with the
Affymetrix GeneChip® Human Gene 1.0 ST Array. Transcripts of 98 genes were up-regulated after differentiation. These genes
could be clustered into subcategories such as cell differentiation, cell morphogenesis, and skeletal development. Osteoblast-specific
transcription factors like osterix and runx2 were constitutively expressed in differentiated DFCs. In contrast, the transcription
factor ZBTB16, which promotes the osteoblastic differentiation of mesenchymal stem cells as an up-stream regulator of runx2,
was differentially expressed after differentiation. Transcription factors NR4A3, KLF9 and TSC22D3, involved in the regulation
of cellular development, were up-regulated as well. In conclusion, we present the first transcriptome of human DFCs before
and after osteogenic differentiation. This study sheds new light on the complex mechanism of osteogenic differentiation in
DFCs.

0 0
 · 
0 Bookmarks
 · 
50 Views
  • Source
    Article: Dental follicle stem cells and tissue engineering.
    Masaki J Honda, Mari Imaizumi, Shuhei Tsuchiya, Christian Morsczeck
    [show abstract] [hide abstract]
    ABSTRACT: Adult stem cells are multipotent and can be induced experimentally to differentiate into various cell lineages. Such cells are therefore a key part of achieving the promise of tissue regeneration. The most studied stem cells are those of the hematopoietic and mesenchymal lineages. Recently, mesenchymal stem cells were demonstrated in dental tissues, including dental pulp, periodontal ligament, and dental follicle. The dental follicle is a loose connective tissue that surrounds the developing tooth. Dental follicle stem cells could therefore be a cell source for mesenchymal stem cells. Indeed, dental follicle is present in impacted teeth, which are commonly extracted and disposed of as medical waste in dental practice. Dental follicle stem cells can be isolated and grown under defined tissue culture conditions, and recent characterization of these stem cells has increased their potential for use in tissue engineering applications, including periodontal and bone regeneration. This review describes current knowledge and recent developments in dental follicle stem cells and their application.
    Journal of Oral Science 01/2010; 52(4):541-52.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

Affymetrix GeneChip® Human Gene 1.0 ST Array
 
cell differentiation
 
cell morphogenesis
 
cellular development
 
complex mechanism
 
dental follicle cells
 
differentiated DFCs
 
first transcriptome
 
genes
 
human dental follicles
 
human DFCs
 
osteoblast-like cells
 
osteoblastic differentiation
 
osteogenic differentiation
 
osteogenic precursor cells
 
real-time RT-PCR results
 
skeletal development
 
study sheds new light
 
Transcription factors NR4A3
 
up-stream regulator