Article

Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

Chinese Journal of Oceanology and Limnology (impact factor: 0.5). 04/2012; 29(4):912-916. DOI:10.1007/s00343-011-0521-9

ABSTRACT An inter-laboratory comparison of the AOAC mouse bioassay for paralytic shellfish poisoning (PSP) toxicity in shellfish was
carried out among 25 Chinese laboratories to examine the overall performance for PSP testing in China, and to analyze the
main factors affecting the performance of this method. The toxic scallop Patinopecten yessoensis collected from coast of Bohai Sea, China, was used as a test sample in the comparison study. The results were reported and
evaluated using robust statistical methods. The z scores showed that 80%, 8%, and 12% of laboratories reported satisfactory results, unsatisfactory results, and questionable
results, respectively. This evaluation demonstrates that the PSP mouse bioassay is an appropriate method for screening and
testing PSP toxicity in shellfish. However, it was found that the experience and skill of technicians, as well as the body
weight and health status of mice being used significantly affected the accuracy of the method.

Keywordparalytic shellfish poisoning (PSP)–mouse bioassay–inter-laboratory comparison

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Keywords

25 Chinese laboratories
 
AOAC mouse bioassay
 
appropriate method
 
Bohai Sea
 
health status
 
inter-laboratory comparison
 
Keywordparalytic shellfish poisoning
 
laboratories
 
paralytic shellfish poisoning
 
PSP mouse bioassay
 
PSP testing
 
PSP)–mouse bioassay–inter-laboratory comparison
 
robust statistical methods
 
satisfactory results
 
technicians
 
testing PSP toxicity
 
toxic scallop Patinopecten yessoensis
 
unsatisfactory results
 
z scores
 

Jijuan Cao