Preparation and Performance Research on Glutathione Molecularly Imprinted Polymers
ABSTRACT Methacrylic acid and ethylene glycol dimethacrylate were respectively used as functional monomer and cross-linker to synthesize
molecularly imprinted polymers (MIPs), for adsorption of glutathione (GSH), named GSH-MIPs. Evaluation of various polymers
by static experiment indicated the optimum ratio of template to functional monomer was 1:4; the optimal concentration and
pH of loading buffer were 5.00–6.50mmol L−1 and 5.0, respectively. By Scatchard analysis, the GSH binding site was found in GSH-MIPs, with K
d and Q
max of 1.96mmolL−1 and 47.19μmolg−1, respectively. The method validated the extraction of GSH from yeast cells samples with the enrichment rate of 76%.
KeywordsMolecularly imprinted polymers–Glutathione–GSH-MIPs–Adsorption conditions–Selectivity–Efficient extraction