Article

Preparation and Performance Research on Glutathione Molecularly Imprinted Polymers

Chromatographia (impact factor: 1.2). 04/2012; 74(5):443-450. DOI:10.1007/s10337-011-2089-0 pp.443-450

ABSTRACT Methacrylic acid and ethylene glycol dimethacrylate were respectively used as functional monomer and cross-linker to synthesize
molecularly imprinted polymers (MIPs), for adsorption of glutathione (GSH), named GSH-MIPs. Evaluation of various polymers
by static experiment indicated the optimum ratio of template to functional monomer was 1:4; the optimal concentration and
pH of loading buffer were 5.00–6.50mmol L−1 and 5.0, respectively. By Scatchard analysis, the GSH binding site was found in GSH-MIPs, with K
d and Q
max of 1.96mmolL−1 and 47.19μmolg−1, respectively. The method validated the extraction of GSH from yeast cells samples with the enrichment rate of 76%.

KeywordsMolecularly imprinted polymers–Glutathione–GSH-MIPs–Adsorption conditions–Selectivity–Efficient extraction

0 0
 · 
0 Bookmarks
 · 
19 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

cross-linker
 
functional monomer
 
glutathione
 
GSH binding site
 
GSH-MIPs
 
KeywordsMolecularly imprinted polymers–Glutathione–GSH-MIPs–Adsorption conditions–Selectivity–Efficient extraction
 
Methacrylic acid
 
method validated
 
MIPs
 
optimal concentration
 
Scatchard analysis
 
static experiment
 
template
 
various polymers
 
yeast cells samples
 

Yanjun Tong