Article

The effect of NaCl on proline accumulation in potato seedlings and calli

Acta Physiologiae Plantarum (impact factor: 1.64). 05/2012; 26(3):263-270. DOI:10.1007/s11738-004-0016-9 pp.263-270

ABSTRACT The effects of salt stress were studied on the accumulation and metabolism of proline and its correlation with Na+ and K+ content in shoots and callus tissue of four potato cultivars, viz., Agria, Kennebec (relatively salt tolerant), Diamant and Ajax (relatively salt sensitive). Na+ and proline contents increased in all cultivars under salt stress. However, K+ and protein contents decreased in response to NaCl treatments. The activities of enzymes involved in proline metabolism,
Δ1-pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) increased and decreased, respectively, in response
to elevated NaCl concentrations. The changes of P5CS and ProDH activities in more salt sensitive cultivars (Diamant, Ajax)
were more than those in the tolerant ones. Then the stimulation of synthesis in combination with a partially increase of protein
proteolysis, a decrease in proline utilization and inhibition of oxidation resulted in high proline contents in seedlings
and calli under salt stress. In callus tissue, reduced growth and cell size may be partially responsible for high proline
accumulation in response to high NaCl levels. However, although the basic proline contents in the seedlings of more salt tolerant
cultivars were higher than the sensitive ones, a clear relationship was not generally observed between accumulation of proline
and salt tolerance in potato.

0 0
 · 
0 Bookmarks
 · 
90 Views
  • Source
    Article: Effect of Salt Stress on Growth, Na(+) Accumulation and Proline Metabolism in Potato (Solanum tuberosum) Cultivars.
    Rinse Jaarsma, Rozemarijn S M de Vries, Albertus H de Boer
    [show abstract] [hide abstract]
    ABSTRACT: Potato (Solanum tuberosum) is a major crop world-wide and the productivity of currently used cultivars is strongly reduced at high soil salt levels. We compared the response of six potato cultivars to increased root NaCl concentrations. Cuttings were grown hydroponically and treated with 0 mM, 60 mM and 180 mM NaCl for one week. Growth reduction on salt was strongest for the cultivars Mozart and Mona Lisa with a severe senescence response at 180 mM NaCl and Mozart barely survived the treatment. The cultivars Desiree and Russett Burbank were more tolerant showing no senescence after salt treatment. A clear difference in Na(+) homeostasis was observed between sensitive and tolerant cultivars. The salt sensitive cultivar Mozart combined low Na(+) levels in root and stem with the highest leaf Na(+) concentration of all cultivars, resulting in a high Na(+) shoot distribution index (SDI) for Mozart as compared to Desiree. Overall, a positive correlation between salt tolerance and stem Na(+) accumulation was found and the SDI for Na(+) points to a role of stem Na(+) accumulation in tolerance. In stem tissue, Mozart accumulated more H2O2 and less proline compared to the tolerant cultivars. Analysis of the expression of proline biosynthesis genes in Mozart and Desiree showed a clear reduction in proline dehydrogenase (PDH) expression in both cultivars and an increase in pyrroline-5-carboxylate synthetase 1 (P5CS1) gene expression in Desiree, but not in Mozart. Taken together, current day commercial cultivars show promising differences in salt tolerance and the results suggest that mechanisms of tolerance reside in the capacity of Na(+) accumulation in stem tissue, resulting in reduced Na(+) transport to the leaves.
    PLoS ONE 01/2013; 8(3):e60183. · 4.09 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

basic proline contents
 
callus tissue
 
cell size
 
clear relationship
 
K+ content
 
NaCl levels
 
potato cultivars
 
ProDH activities
 
proline contents
 
proline dehydrogenase
 
proline metabolism
 
proline utilization
 
protein contents
 
salt sensitive
 
salt sensitive cultivars
 
salt stress
 
salt tolerant
 
sensitive ones
 
tolerant ones
 
Δ1-pyrroline-5-carboxylate synthetase
 

H. Rahnama