Article

Hepatic nitrosative stress in experimental diabetes.

Laboratório de Fisiologia e Gastroenterologia Experimental, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS) 90035-903 Porto Alegre, RS, Brazil; Curso de Fisioterapia/Centro de Ciências da Vida e da saúde - Universidade Católica de Pelotas (UCPEL) 96010-000, Pelotas, RS, Brazil.
Journal of diabetes and its complications (impact factor: 2.11). 06/2012; 26(5):378-81. DOI:10.1016/j.jdiacomp.2012.04.015 pp.378-81
Source: PubMed

ABSTRACT The effects of the inhibition of nitrosative stress by aminoguanidine in an experimental model of diabetes mellitus (DM) were investigated.
Twenty-one male Wistar rats were divided into three groups: control (CO), diabetic (DM), and diabetic treated with aminoguanidine (DM+AG). Aminoguanidine (aminoguanidine hemisulfate salt, Sigma Chemical Co., St. Louis, MO, USA) was used at a dose of 50mg/kg (i.p.) during the last 30days of the experiment. The expression levels of liver lipoperoxidation (TBARS - nmol/mg protein), inducible oxide nitric synthase (iNOS), nitrotyrosine and the NFκB nuclear transcription factor p65 were examined using western blot analysis.
The DM group demonstrated an increase in lipoperoxidation and in the expression of iNOS, nitrotyrosine and p65. Aminoguanidine reduced hepatic lipid peroxidation and protein expression levels of iNOS, nitrotyrosine and p65.
Aminoguanidine treatment reduces liver oxidative and nitrosative stress in diabetic animals. In addition, aminoguanidine reduced the expression of p65 in the liver.

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Keywords

aminoguanidine hemisulfate salt
 
Aminoguanidine treatment
 
diabetes mellitus
 
diabetic
 
diabetic animals
 
DM group
 
DM+AG
 
experimental model
 
expression levels
 
hepatic lipid peroxidation
 
inducible oxide nitric synthase
 
inhibition
 
last 30days
 
liver lipoperoxidation
 
male Wistar rats
 
NFκB nuclear transcription factor p65
 
nitrosative stress
 
nmol/mg protein
 
protein expression levels
 
western blot analysis