Eight microsatellite loci were identified and characterized for the endangered Machilus pseudokobu (Lauraceae), an endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from 1 to
20 with an average of 6.2, and the expected heterozygosity ranged from 0.00 to 0.83 with an average of 0.47. All eight loci
were screened in cross-amplification tests for two other endemic Machilus species that also inhabit the Bonin Islands. All loci were successfully amplified in these species.
" dry forest ) , 19 in Higashid - aira ( mesic forest ) , 23 in Tatsumi ( mesic forest ) , and 19 in Yoake - yama ( mesic forest ) . We extracted genomic DNA from the plant tissue using modifications of the Cetyl trimethyl ammonium bromide ( CTAB ) method ( Doyle & Doyle 1987 ) and analysed seven microsatellite markers we had developed previously ( Tsuneki et al . 2009 ) . All poly - merase chain reaction ( PCR ) products were subjected to capillary electrophoresis and fragment analysis on an ABI PRISM 3100 Genetic Analyzer ( Applied Biosystems , Foster City , CA ) . The size of the amplified fragments was scored automatically using GeneMapper 4 . 0 software ( Applied Biosystems , Foster City , CA ) a"
[Show abstract][Hide abstract] ABSTRACT: Persea boninensis (Lauraceae) is an endemic tree species distributed throughout the Bonin Islands. It grows in a wide range of environments from dry to mesic forests, and has multiple flowering peaks that may correspond to different habitats on Chichijima Island of the Bonin Islands. We predicted that P. boninensis is differentiated into two groups with different habitats on these islands. We examined and compared the flowering phenology, morphology, and genetics of populations of species growing in dry and mesic forests. We also performed preliminary artificial crossing experiments. Based on our results, P. boninensis on the Chichijima Islands can be clearly divided into two genetic groups with different habitats and flowering times. Although the flowering time difference could act as an effective pre-zygotic isolation mechanism between the two groups, there was still a 1-month overlap in flowering time. Furthermore, our artificial crossing experiments between the two groups resulted in plants that set seeds. Therefore, there was no evidence of reproductive isolation after fertilization. Differences in flowering time as well as in growth habitat will have to occur to maintain genetic differentiation between the two groups of P. boninensis.
Plant Species Biology 01/2014; 29(1). DOI:10.1111/j.1442-1984.2012.00383.x · 1.27 Impact Factor
"Because microsatellite loci are hypervariable and reproducible markers, they have been widely used for population, systematics, and conservation studies (Pardo et al. 2008; Hiraoka and Tomaru 2009; Honjo et al. 2009). Such loci are actively being developed for plant species (King and Roalson 2009; Nakagawa and Ito 2009; Tsuneki et al. 2009). Quercus acuta is distributed only in Korea and Japan (Ohashi et al. 2006a) and grows in warm-temperate evergreen broadleaf forests. "
[Show abstract][Hide abstract] ABSTRACT: Quercus acuta is an evergreen broadleaf tree that grows in the warm-temperate regions of Korea and Japan. Its habitats and populations
are being destroyed, and a new northernmost limit of distribution has now been reported. To further our scientific understanding
of its conservation and phylogeography, we isolated and characterized 13 microsatellite loci. An analysis of diversity was
conducted among 35 individuals on Hong-do Island of Jeollanam-do, South Korea. Variability of the markers was also tested
for 11 individuals from Jeju-do. At the population level, alleles numbered 2 to 12 and the observed and expected heterozygosities
ranged from 0.0909 to 0.9143 and from 0.0909 to 0.9364, respectively. Those 13 loci were also tested for cross-species amplification
in three other evergreen Quercus species within the same subgenus Cyclobalanopsis. In all, 6 of 13 loci could be amplified for all three species. The microsatellite markers described here provide a powerful
genetics tool for population, conservation, systematics, and phylogeographic studies, not only for Q. acuta but also for other evergreen Quercus species.
KeywordsEvergreen broadleaf tree-Marker-Microsatellite-Phylogeography-Subgenus Cyclobalanopsis
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