Chemiluminescence detection with separation techniques for bioanalytical applications
ABSTRACT Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with
separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis.
This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques
published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemiluminescence
have been mainly used for bioanalytical application. In this paper, only the applications of the method for the analysis of
biosamples, serum, plasma, urine, and tissue samples are discussed.
Article: Simultaneous determination of four different antibiotic residues in honey by chemiluminescence multianalyte chip immunoassays[show abstract] [hide abstract]
ABSTRACT: We are presenting the first method for identification and quantification of antibiotic derivatives in honey samples using regenerable antigen microarrays in combination with an automated flow injection system. The scheme is based on an indirect competitive immunoassay format using monoclonal antibodies bound to the surface of the microarray. The surface of glass slides was coated with epoxy-activated poly(ethylene glycol) and enables direct immobilization of the antibiotic derivatives. The antigen/antibody interaction on the surface of the chip can be detected by chemiluminescence (CL) read-out via CCD camera. The method allows for fast analysis of the four analytes simultaneously and without purification or extraction. An effective data evaluation method also was developed to warrant unambiguous identification of the spots and to establish grey levels of CL intensities. The software developed enables fast and automated processing of the CL images. Dose–response curves were obtained for the derivatives of enrofloxacin, sulfadiazine, sulfamethazine and streptomycin. Spiking experiments revealed adequate recoveries within the dynamic ranges of the calibration curves of enrofloxacin (92% ± 6%), sulfamethazine (130% ± 21%), sulfadiazine (89% ± 20%) and streptomycin (93% ± 4%). FigureFlow-scheme of the chemiluminescence multianalyte chip immunoassay for the determination of antibiotic residues in honey KeywordsAntibiotic microarray–Chemiluminescence detection–Regenerable biochip–Automated flow-injection system–Microarray image evaluationMicrochimica Acta 04/2012; 173(1):1-9. · 3.03 Impact Factor