Chemiluminescence detection with separation techniques for bioanalytical applications
ABSTRACT Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with
separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis.
This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques
published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemiluminescence
have been mainly used for bioanalytical application. In this paper, only the applications of the method for the analysis of
biosamples, serum, plasma, urine, and tissue samples are discussed.
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ABSTRACT: We are presenting the first method for identification and quantification of antibiotic derivatives in honey samples using regenerable antigen microarrays in combination with an automated flow injection system. The scheme is based on an indirect competitive immunoassay format using monoclonal antibodies bound to the surface of the microarray. The surface of glass slides was coated with epoxy-activated poly(ethylene glycol) and enables direct immobilization of the antibiotic derivatives. The antigen/antibody interaction on the surface of the chip can be detected by chemiluminescence (CL) read-out via CCD camera. The method allows for fast analysis of the four analytes simultaneously and without purification or extraction. An effective data evaluation method also was developed to warrant unambiguous identification of the spots and to establish grey levels of CL intensities. The software developed enables fast and automated processing of the CL images. Dose–response curves were obtained for the derivatives of enrofloxacin, sulfadiazine, sulfamethazine and streptomycin. Spiking experiments revealed adequate recoveries within the dynamic ranges of the calibration curves of enrofloxacin (92% ± 6%), sulfamethazine (130% ± 21%), sulfadiazine (89% ± 20%) and streptomycin (93% ± 4%). FigureFlow-scheme of the chemiluminescence multianalyte chip immunoassay for the determination of antibiotic residues in honey KeywordsAntibiotic microarray–Chemiluminescence detection–Regenerable biochip–Automated flow-injection system–Microarray image evaluationMicrochimica Acta 04/2011; 173(1):1-9. DOI:10.1007/s00604-011-0548-9 · 3.74 Impact Factor
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ABSTRACT: The thermal decomposition of kaolinite was studied by differential thermogravimetry (DTG) technique under non-isothermal conditions. Samples of industrially treated (washed) kaolin with high content of the medium ordered kaolinite were calcined using a heating rate from 1 to 40 K min− 1. The apparent activation energy and frequency factor for the dehydroxylation of kaolinite was evaluated by Kissinger method as 195 ± 2 kJ × mol− 1 and (8.58 ± 0.33) × 1014 s− 1, respectively. Avrami exponent of the process was estimated using Kissinger empirical kinetic models and Carne equation.Graphical AbstractThe thermal decomposition of kaolinite was studied by DTG technique under non-isothermal conditions using heating rate from 1 to 40 K min− 1. The apparent activation energy and pre-exponential factor of process are 195 kJ × mol− 1 and 8.58 × 1014 s− 1, respectively. The value of Avrami exponent was estimated using Kissinger empirical kinetic models and Carne equation.Research Highlights► The dehydroxylation of kaolinite was studied by DTG under non-isothermal conditions. ► Avrami exponent was estimated using Kissinger kinetic models and Carne equation. ► The apparent activation energy was evaluated by Kissinger method as 195 ± 2 kJ × mol− 1. ► Apparent kinetic exponent corresponds to the diffusion controlled growth.Powder Technology 03/2011; 208(1):20-25. DOI:10.1016/j.powtec.2010.11.035 · 2.35 Impact Factor
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ABSTRACT: Menadione (2-methyl-1,4-naphthoquinone, MQ), a component of multivitamin drugs with antihemorrhagic, antineoplastic, and antimalarial activity, is frequently used to investigate quinone-induced cytotoxicity. The formation of MQ conjugates with glutathione (GSH) by Michael addition and subsequent biotransformation to yield N-acetyl-L-cysteine conjugates is believed to be an important detoxification process. However, the resulting conjugates, 2-methyl-3-(glutathione-S-yl)-1,4-naphthoquinone (MQ-GS) and 2-methyl-3-(N-acetyl-L-cysteine-S-yl)-1,4-naphthoquinone (MQ-NAC), retain the ability to redox cycle and to arylate cellular nucleophiles. Although the nephrotoxicity and hepatotoxicity of MQ-thiol conjugates have been reported in vitro, methods for their determination in vivo have yet to be published. Herein, a highly sensitive, simple, and selective HPLC-chemiluminescence (HPLC-CL) coupled method is reported, allowing for the first time the simultaneous determination of MQ, MQ-GS and MQ-NAC in rat plasma after MQ administration. Our method exploits the unique redox characteristics of MQ, MQ-GS and MQ-NAC to react with dithiothreitol (DTT) to liberate reactive oxygen species (ROS) which are detected by a CL assay using luminol as a CL probe. To verify the proposed mechanism, MQ-GS and MQ-NAC were synthetically prepared. Specimen preparation involved solid-phase extraction on an Oasis HLB cartridge followed by isocratic elution on an ODS column. No interference from endogenous substances was detected. Linearity was observed in the range of 5-120 nM for MQ-GS and MQ-NAC and 10-240 nM for MQ, with detection limits (S/N of 3) of 1.4, 0.8 and 128 fmol for MQ-GS, MQ-NAC and MQ, respectively. The application of our method reported here is the first to extensively study the stability and reversibility of thiol-quinones.Chemical Research in Toxicology 08/2013; 26(9). DOI:10.1021/tx400253k · 3.53 Impact Factor