Determination of organic acids evolution during apple cider fermentation using an improved HPLC analysis method

European Food Research and Technology (Impact Factor: 1.39). 08/2008; 227(4):1183-1190. DOI: 10.1007/s00217-008-0835-9

ABSTRACT An efficient method for analyzing ten organic acids in food, namely citric, pyruvic, malic, lactic, succinic, formic, acetic,
adipic, propionic and butyric acids, using HPLC was developed. Boric acid was added into the mobile phase to separate lactic
and succinic acids, and a post-column buffer solution [5mmol/L p-toluensulfonic acid (p-TSA)+20mmol/L bis (2-hydroxyethyl) iminotris (hydroxymethyl) methane (bis–tris)+100μmol/L sodium ethylenediaminetetraacetic
(EDTA-2Na)] was used to improve the sensitivity of detection. The average spiked recoveries for the ten organic acids ranged
from 82.9 to 127.9% with relative standard deviations of 1.44–4.71%. The linear ranges of determination were from 15 to 1,000mg/L
with correlation coefficients of 0.9995–0.9999. The metabolism of organic acids in cider, and the effect of nutrients including
diammonium phosphate (DAP), thiamine, biotin, niacinamide and pantothenic acid on their metabolism, were studied using this
method of analysis. We found that before cider brewing, additions of 200mg/L DAP and 0.3mg/L thiamine to apple juice concentrate
results in a high quality cider.

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