Dye-enhanced laser fluorescence detection of caries lesions around brackets

Lasers in Medical Science (Impact Factor: 2.49). 11/2009; 24(6):865-870. DOI: 10.1007/s10103-008-0572-0


The aim was to evaluate the performance of DIAGNOdent [laser fluorescence(LF) and LFpen] devices enhanced by fluorescent dye
in detecting mineral loss around brackets and comparing the inhibitory effect of bonding material on artificial demineralization,
and to verify whether LF methods show the same trends of mineral loss. Brackets were bonded to premolar halves with Fuji Ortho
LC, Transbond XT, and Ortho Glass LC cements (n = 15). The teeth were soaked in demineralizing solution (pH = 4.8) for 16days. Mineral loss was calculated by atomic emission
spectrometry, and lesions were measured with LF devices with dye [tetrakis N-methylpyridyl porphyrin (TMPyP)]. Groups were compared with regard to LF readings and mineral loss, and performance of caries
detection was calculated. Higher mineral loss and LF-TMPyP values occurred in the resin group. LFpen-TMPyP readings were significantly
higher in the demineralized groups. Correlation was observed between mineral loss and LF measurements. LF methods are capable
of identifying lower demineralization around brackets bonded with resin-modified glass ionomer cements.

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    • "The first group served as the control, whereas the teeth in other groups were immersed for 16 weeks in a cariogenic solution to produce demineralised enamel. This solution consisted of 2.2 mM CaCl2, 2.2 mM NaH2PO4 and 50 mM acetic acid, with pH adjusted to 4.8 using KOH [24]. The specimens were immersed individually in plastic containers with approximately 10 ml of cariogenic solution, and the solution was replaced weekly. "
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    ABSTRACT: The aim of this investigation was to assess the effects of different treatments of demineralised enamel on microleakage under orthodontic brackets. Seventy-five intact premolars were randomly assigned to five groups. The teeth in groups 2 through 5 were immersed in a demineralising solution for 16 weeks. In groups 1 (control) and 2 (demineralised/control), conventional acid etching was used. In group 3, sodium hypochlorite (NaOCl) was applied on the enamel surface for 1 min after acid etching, and in group 4, Transbond Plus (3M Unitek, Monrovia, CA, USA) self-etching primer (SEP) was used. The teeth in group 5 were treated with 2% sodium fluoride (NaF) for 4 min before etching. After bracket bonding, the specimens were thermocycled, sealed with nail varnish, immersed in 0.5% basic fuchsine solution for 24 h and sectioned. Microleakage was measured under a stereomicroscope for the enamel-adhesive and adhesive-bracket interfaces of both occlusal and gingival sides. Demineralised teeth showed more microleakage at the enamel-adhesive interface on both occlusal and gingival sides compared to sound teeth, but the difference was not significant (P > 0.005). Treating the demineralised enamel with 5% NaOCl or Transbond Plus SEP was not effective in reducing microleakage. NaF treatment followed by acid etching of demineralised enamel resulted in significantly lower microleakage in most comparisons (P < 0.005). The use of 2% NaF on hypomineralised enamel before the bracket bonding procedure is an effective way to decrease microleakage.
    05/2013; 14(1):2. DOI:10.1186/2196-1042-14-2
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    ABSTRACT: The aims of this in vitro study were (1) to compare the reliability of a new fluorescence camera (FC; VistaProof) with that of two other laser fluorescence devices (DIAGNOdent and DIAGNOdent pen) in assessing smooth and occlusal caries lesions in primary teeth and (2) to determine the influence of the spacer, the protective cover and air drying on the FC readings. We selected 129 primary molars which were divided into two groups: smooth surfaces (n=68) and occlusal surfaces (n=61). Two trained examiners performed the evaluations using the FC and the other laser fluorescence devices separately. One examiner repeated all assessments after 1 week. Inter- and intraexaminer reproducibility was calculated from the absolute values derived from the devices using intraclass correlation coefficient (ICC) and Bland-Altman analysis. Pearson correlation was performed among the values obtained with the different devices. For aim 2, only teeth from the occlusal surface group were used. Another examiner performed measurements with the FC, varying the size of the spacer, the use of the plastic protective cover and the drying time (0, 3 and 15 s). Wilcoxon's test was used to compare these measurements. High values of interexaminer ICC were observed with the FC in both experiments. However, the highest intraexaminer ICC for smooth surfaces was found for the DIAGNOdent (ICC 0.918). A positive correlation between all methods was observed in both studies. Only drying for 15 s significantly increased the FC readings. In conclusion, the FC showed high reliability for occlusal and smooth surfaces, similar to the other fluorescence methods tested. Additionally, prolonged air drying increased the FC measurements.
    Lasers in Medical Science 02/2010; 26(2):157-62. DOI:10.1007/s10103-010-0757-1 · 2.49 Impact Factor
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    Principles in Contemporary Orthodontics, 11/2011; , ISBN: 978-953-307-687-4
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