Estimating agronomic traits of transgenic tobacco lines
ABSTRACT Tobacco transgenic lines belonging to four cultivars modified with three genetic constructions providing the resistance to
Potato virus Y (PVY) had been described as regards their agronomic and biological traits. The constructions included viral genes: the coat
protein gene of Lettuce mosaic virus (LMV) and the replicase gene of PVY used in sense and antisense orientation. Evaluation of the chosen farm traits of the
transgenic tobacco lines was performed by comparing them with their nontransgenic equivalents. The traits such as growth and
development rate, height of stalks, leaf area, morphology of plants, course of meiosis and pollen viability were described.
The cured leaves were analysed as regards to their physical traits and chemical composition. The individual transgenic lines
differed from control cultivars to a little extent. Only insignificant changes, mainly in the quality of raw material were
observed. Genetic modification did not cause any changes in the course of meiosis and pollen viability.
Potato virus Y
- Crop Science - CROP SCI. 01/1999; 39(4).
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ABSTRACT: Transgene technology provides a powerful tool for developing traits that are otherwise difficult to achieve through conventional breeding. In order to effectively apply the technology to breeding, we need to understand how transgenes behave in plants. Transgenes may or may not follow Mendelian segregation; their expression can be significantly affected by integration positions and structures of the transgenic DNA in host genomes; transgenes may become unstable over generations, genetic background sand environmental conditions; and they may have significantly negative impact on expression of endogenous genes. If not well understood, the sehurdles could become significant barriers in transgenic breeding. This paper reviews some genetic issues and pitfalls that are often encountered in transgenic breeding. Because of the necessity of being brief, transgene expression, silencing, and breeding are the three areas of focuses in this discussion. While molecular mechanisms underlying many of the transgenic phenomena have not been completely understood, some practical ‘rules’ are now available for creating, evaluating and selecting desirable transgenic transformants. It can be certain that with more transgenic plants generated and characterized our knowledge of transgene genetics at both molecular and plant levels will continue to accumulate.Euphytica 04/2012; 118(2):137-144. · 1.64 Impact Factor
- Crop Science - CROP SCI. 01/2003; 43(2).