Mesenchymal stem cells derived from different origins have unique sensitivities to different chemotherapeutic agents.
ABSTRACT Bone-marrow-derived mesenchymal stem cells（BMSCs）and adipose-tissue-derived mesenchymal stem cells (ADSCs）are virtually identical in cell surface marker profile and differentiation potential. These cell populations have promising characteristics for clinical application. In this study, we evaluated the sensitivity of these cell populations to various chemotherapeutic agents by testing the inhibition of cell proliferation, low molecular DNA bands formation, in situ apoptosis, apoptosis related gene expression and cell senescence after treatment with these agents. Our results demonstrated that busulfan, methotrexate and doxorubicin treatments led to a marked and dose-dependent reduction in cell viability when compared with 5-fluorouracil treatment. Different expression patterns of apoptosis-related genes were found in the BMSCs and ADSCs following treatment with the agents, but no low molecular weight DNA bands were detected. BMSCs demonstrated a higher percentage of apoptotic and senescent cells following treatment with chemotherapeutic agents compared with ADSCs. These findings suggest that these two cell populations respond differently to chemotherapy treatment, ADSCs exhibit more resistant than BMSCs to chemotherapy treatment induced cell senescence and apoptosis, indicating it might be more advantageous to use in the clinic than BMSCs.
- SourceAvailable from: Mahmoud Hashemitabar[show abstract] [hide abstract]
ABSTRACT: In recent years, examination and comparison of the biological characteristics of bone marrow- and adipose-derived mesenchymal stem cells (MSCs) from various perspectives have come into the focus of stem cell research, as these cells should be well characterized in order to utilize them in future cellular therapies. Therefore, in the present study, surface protein markers and the skeletal myogenic differentiation potential of rat bone marrow- and adipose-derived MSCs were examined. The expression of CD44, CD45, CD73, and CD90 on bone marrow- and adipose-derived MSCs was characterized using flow cytometry. Subsequently, the stem cells were differentiated into myogenic lineages, and the expression of the skeletal myogenic markers MyoD1, Myog, and Myh2 was studied in cells using real time polymerase chain reaction and immunofluorescence. Our results reveal that the pattern of CD marker expression differs between these 2 types of MSCs to some extent, whereas no significant difference was observed with respect to their myogenic differentiation potential. Therefore, we concluded that despite the differences observed in the biological features of these 2 types of MSCs, their myogenic potential appears to be similar, and that adipose-derived stem cells may be useful in skeletal muscle tissue engineering, due to their easy isolation and capacity for rapid expansion in a short time span.Anatomy & cell biology 05/2013;